Evgenia D. Moiseeva

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Uncovering the nature of communication between enhancers, promoters and insulators is important for understanding the fundamental mechanisms that ensure appropriate gene expression levels. Here we describe an approach employing transient expression of genetic luciferase reporter gene constructs with quantitative RT-PCR analysis of transcription between an(More)
217 In recent years, convincing evidences have been presented demonstrating that eukaryotic chromosomes contain extended regions in which regulation of gene transcription is coordinated—gene expression is switched on or off. Such chromosomal regions are called transcriptional territories [1]. The latter were discovered using the microarray technique.(More)
60 Transcriptional regulation of many eukaryotic genes is ensured by regulatory elements, which are often located at significant distances from their pro moters. The main classes of such elements are enhancers, locus control regions, insulators, and PRE/TRE regions. In complex genetic loci, various distantly acting regulatory elements that sometimes are(More)
102 According to modern ideas, coordinated regula tion of gene expression within large genetic loci of Drosophila is mediated by PRE and TRE DNA sequences, which can specifically interact with pro teins from Polycomb (PcG) and Trithorax (TrxG) groups, respectively. PcG proteins function as repres sors, whereas TrxG proteins are activators [1, 2]. PRE/TRE(More)
In the present paper, the interactions of the origin binding protein (OBP) of herpes simplex virus type 1 (HSV1) with synthetic four-way Holliday junctions (HJs) were studied using electrophoresis mobility shift assay and the FRET method and compared with the interactions of the protein with duplex and single-stranded DNAs. It has been found that OBP(More)
342 The eukaryotic genome consists of structural–functional units 50–200 kb long, containing various genes or large genetic loci that are concertedly silenced or activated. These units were termed forum domains [1– 3]. The conclusion on the existence of such domains was independently confirmed by Tolhuis et al. [4], who called them Pc domains. The(More)
The binding of distamycin dimeric analog (Pt-bis-Dst) to poly[d(A-T)] x poly[d(A-T)1, poly(dA) x poly(dT) and duplex O23 with the sequence 5'-GCCAATATATATATATTATTAGG-3' which is present at the origin of replication of herpes simplex virus OriS is investigated with the use of UV and CD spectroscopy. The distinction of the synthetic polyamide from a natural(More)
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