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Maximization of growth rate is an important fitness strategy for bacteria. Bacteria can achieve this by expressing proteins at optimal concentrations, such that resources are not wasted. This is exemplified for Escherichia coli by the increase of its ribosomal protein-fraction with growth rate, which precisely matches the increased protein synthesis demand.(More)
Evolutionary adaptations in metabolic networks are fundamental to evolution of microbial growth. Studies on unneeded-protein synthesis indicate reductions in fitness upon nonfunctional protein synthesis, showing that cell growth is limited by constraints acting on cellular protein content. Here, we present a theory for optimal metabolic enzyme activity when(More)
Protein expression is shaped by evolutionary processes that tune microbial fitness. The limited biosynthetic capacity of a cell constrains protein expression and forces the cell to carefully manage its protein economy. In a chemostat, the physiology of the cell feeds back on the growth conditions, hindering intuitive understanding of how changes in protein(More)
Evolutionary pressures on microbial metabolic strategies in the chemostat In collaboration with: Abstract Protein expression is shaped by evolutionary pressures. Due to limitations in biosynthetic capacity , the costs and benefits of enzyme production are important determinants of fitness. While these processes are well understood in batch conditions, in(More)
Microorganisms rely on binding-protein assisted, active transport systems to scavenge for scarce nutrients. Several advantages of using binding proteins in such uptake systems have been proposed. However, a systematic, rigorous and quantitative analysis of the function of binding proteins is lacking. By combining knowledge of selection pressure and(More)
Low affinity membrane transporters increase the net substrate uptake rate by reducing substrate efflux In collaboration with: Abstract Cells require membrane-located transporter proteins to import energy and carbon sources from the environment. Many organisms have several similar transporters for the same nutrient, which differ in their affinity. Typically,(More)
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