Learn More
In this study, myosin types in human skeletal muscle fibers were investigated with electrophoretic techniques. Single fibers were dissected out of lyophilized surgical biopsies and typed by staining for myofibrillar ATPase after preincubation in acid or alkaline buffers. After 14C-labelling of the fiber proteins in vitro by reductive methylation, the myosin(More)
The immunological properties of myosins, especially their muscle-type, class and species specificity, are still controversial. It is the opinion of the authors that the lack of agreement might at least in part be due to the use of contaminated myosins as immunogens and inappropriate methods. We, therefore, purified myosins to a very high degree(More)
The fibre type IIM, present in the jaw-closer muscles of the cat, has a characteristic form of myosin. To analyse other proteins present in IIM fibres and the usual types I, IIA and IIB, 14C-labelled typed single fibres were analysed by 2-dimensional gel electrophoresis. Whereas types I, IIA and IIB contained both alpha- and beta-forms of tropomyosin, IIM(More)
The effect of transferrins on hydroxyl radical formation from the superoxide anion and hydrogen peroxide generated by the xanthine-xanthine oxidase system has been studied by EPR using 5,5-dimethyl-1-pyrroline N-oxide as a spin trap. Neither diferriclactoferrin nor diferrictransferrin were found capable of promoting hydroxyl radical formation via the(More)
There is good evidence for the coexistence of different myosin types both in developing muscles and in Purkinje cells from adult chicken hearts. In skeletal muscle fibres of adult animals, however, coexistence of fast (FM) and slow (SM) myosin has only been demonstrated after long-term electrical stimulation. The term 'promiscuity' has recently been coined(More)
AIMS To quantify and to compare the occurrence of Fusarium species in maize kernels and stalk pieces, to analyse mycotoxins in kernels and maize crop residues, to evaluate two approaches to obtain kernel samples and to compare two methods for mycotoxin analyses. METHODS AND RESULTS The occurrence of Fusarium species in maize kernels and stalk pieces from(More)
We examined the relationships of the genomes of five strains of Borrelia burgdorferi isolated from ticks, two from North America, including the type strain B31, and three from Switzerland. We determined restriction fragment length polymorphisms by using eight cloned DNA fragments as hybridization probes to genomic Southern blots. Two divergent patterns were(More)
Chicken muscle beta-actinin is considered to be one of the "true" myofibrillar components due to its specific binding to isolated myofibrils. Surprisingly, the direct comparison of this muscle protein with serum albumin, both isolated from chicken, showed that they behaved identically under several electrophoretic conditions. Furthermore, immunoreplica gels(More)