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Cardiolipin Prevents Membrane Translocation and Permeabilization by Daptomycin*
The collective findings lead to propose that a daptomycin pore consists of two aligned tetramers in opposite leaflets and that cardiolipin prevents the translocation of tetramer to the inner leaflet, thereby forestalling the formation of complete, octameric pores. Expand
First synthesis of free cholesterol-BODIPY conjugates.
An analogue of cholesterol and coprostanol containing the BODIPY fluorophore in the aliphatic tail of the free sterol exhibited a substantially similar physical behavior to cholesterol in model membranes with respect to localization in raft domains. Expand
Behavior of a photoactivatable analog of cholesterol, 6‐photocholesterol, in model membranes
The ability of this probe, under conditions in which it is not photoactivated to a carbene, to substitute for cholesterol in two unrelated assays is analyzed, suggesting that this analog is a suitable photoprobe of cholesterol. Expand
Synthesis and characterization of PEGylated bolaamphiphiles with enhanced retention in liposomes.
Long-circulating liposomes are typically prepared with poly(ethylene glycol)- (PEG-) modified lipids, where the lipid portion is inserted in the lipid bilayers as an anchor and the hydrophilic PEGExpand
Two successive calcium-dependent transitions mediate membrane binding and oligomerization of daptomycin and the related antibiotic A54145.
These findings agree with the earlier observation that two of the four acidic amino acid residues in the daptomycin molecule are essential for antibacterial activity. Expand
Interaction of two oxysterols, 7-ketocholesterol and 25-hydroxycholesterol, with phosphatidylcholine and sphingomyelin in model membranes.
The ability of oxysterols to condense phosphatidylcholine and sphingomyelin films, their capacity to cause changes in in-plane elasticity moduli, and their propensity to form detergent-resistant membrane domains were all found to be dependant on the location of the oxygen functionality in the oxysterol and the chemical nature of the phospholipid in the model systems. Expand
The critical micelle concentrations of lysophosphatidic acid and sphingosylphosphorylcholine.
The critical micelle concentrations (CMC) of lysophosphatidic acid (LPA) and sphingosylphosphorylcholine (SPC) were measured by isothermal titration calorimetry and did not change with an increase in salt concentration. Expand
Lysophosphatidic acid and lipopolysaccharide bind to the PIP2-binding domain of gelsolin.
The binding of the gelsolin P2 peptide (residues 150-169) with lysophosphatidic acid (LPA) and lipopolysaccharide (LPS) was investigated by isothermal titration calorimetry. P2 binds to LPS withExpand
Mutual inhibition through hybrid oligomer formation of daptomycin and the semisynthetic lipopeptide antibiotic CB-182,462.
The existence of functionally impaired oligomers indicates that oligomers formation is indeed important for antibacterial function, but it also shows that oligomerization is not sufficient; once formed, the oligomers must take another step in order to acquire antibacterial activity. Expand
Differential effects of conjugated linoleic acid isomers on the biophysical and biochemical properties of model membranes.
The results suggest that the differences in the biophysical properties of CLA isomers A and C may partly contribute to the known differences in their biological effects. Expand