Eva Schraeder

Learn More
For the improved production of vaccines and therapeutic proteins, a detailed understanding of the metabolic dynamics during batch or fed-batch production is requested. To study the new human cell line AGE1.HN, a flexible metabolic flux analysis method was developed that is considering dynamic changes in growth and metabolism during cultivation. This method(More)
Background With the emergence of functional genomics approaches in the past decade, powerful tools for the discovery and understanding of cellular mechanisms are accessible. The main purpose of those analyses is to investigate the cell on the level of the transcriptome, proteome and metabolome to retrieve information on possible limitations for growth or(More)
Background Human cell lines are an interesting alternative to CHO cells for the production of recombinant proteins and monoclonal antibodies, because of their ability to produce genuine human posttranslational modifications. The human cell line AGE1.HN.AAT (ProBioGen, Berlin, Germany), that originated from human neural precursor tissue, has been adapted to(More)
Background Development of bioprocesses for animal cells has to deal with different bioreactor types and scales. Bioreactors might be intended for generation of cell inoculum and production, research, process development, validation or transfer purposes. During these activities, not only the difficulty of upand downscaling might lead to failure of(More)
Background In Systems Biology approaches a good amount of reliable data is essential for effective modelling of distinct biological processes. Hence the focus of the SysLogicsProject was on modelling of the central metabolism using different functional genomic techniques. For displaying the involved proteins, it is crucial to enrich them using subproteomic(More)
  • 1