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BACKGROUND Platelet-derived microparticles (PMPs) are generally considered a marker of platelet activation in cardiovascular disease. We studied the extent to which PMP subpopulations parallel platelet activation in vitro and in vivo. METHODS Using flow cytometry, we analyzed PMP subpopulations from resting and activated platelets in vitro (n = 6) as well(More)
BACKGROUND The processes that govern the distribution of molecules between platelets and the microparticles (MP) they release are unknown. Certain proteins are sorted selectively into MP, but lipid sorting has not been studied. OBJECTIVES To compare the phospholipid composition and cholesterol content of platelet-derived MP obtained with various stimuli(More)
BACKGROUND Circulating microparticles of various cell types are present in healthy individuals and, in varying numbers and antigenic composition, in various disease states. To what extent these microparticles contribute to coagulation in vivo is unknown. OBJECTIVES To examine the in vivo thrombogenicity of human microparticles. METHODS Microparticles(More)
OBJECTIVES In vitro, microparticles can activate complement via the classical pathway. If demonstrable ex vivo, this mechanism may contribute to the pathogenesis of rheumatoid arthritis (RA). We therefore investigated the presence of activated complement components and complement activator molecules on the surface of cell-derived microparticles of RA(More)
OBJECTIVES To investigate whether cell-derived microparticles play a role in complement activation in pericardial blood of patients undergoing cardiac surgery with cardiopulmonary bypass (CPB) and whether microparticles in pericardial blood contribute to systemic complement activation upon retransfusion. METHODS Pericardial blood of 13 patients was(More)
Chick-quail chimeric studies were made to determine the origin of the cells of splenic ellipsoid. The ellipsoid is formed by supporting and phagocytic cells, which are embedded in a well-organized extracellular matrix. Splenic and bursal anlage of 6- to 6.5-day-old quail embryos were transplanted into the coelomic cavity of 3-day-old chick embryos and(More)
BACKGROUND Inflammation plays a major role in the vascular dysfunction seen in preeclampsia, and several studies suggest involvement of the complement system. OBJECTIVES To investigate whether complement activation on the surface of microparticles is increased in plasma of preeclamptic patients versus healthy pregnant controls. METHODS Microparticles(More)
BACKGROUND In Fabry disease, storage of globotriaosylceramide (Gb3) in arterial walls is one of the main pathogenetic factors that are thought to underlie the clinical manifestations of the disease. Abnormalities of the vessel wall, haemodynamics and pro- and anticoagulant factors may play a role, though the exact pathophysiology is incompletely understood.(More)
INTRODUCTION Microparticles from activated endothelial cells (EMP) are well known to expose tissue factor (TF) and initiate coagulation in vitro. TF coagulant activity is critically dependent on the presence of aminophospholipids, such as phosphatidylserine (PS) and phosphatidylethanolamine (PE), but it is unknown whether or not TF-exposing EMP are enriched(More)