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Cloning and characterization of a novel thermostable esterase from Bacillus gelatini KACC 12197.
A novel gene encoding a thermostable esterase (designated as Est-gela) was isolated from the moderate thermophile Bacillus gelatini KACC 12197. The open reading frame of this gene (1170 bp) encodesExpand
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Improved 1,3-propanediol production by Escherichia coli from glycerol due to Co-expression of glycerol dehydratase reactivation factors and succinate addition
Escherichia coli was engineered to produce 1,3-propanediol (1,3-PDO) from glycerol, an inexpensive carbon source. This was done by introducing a synthetic pathway consisting of glycerol dehydratase,Expand
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Isolation of microorganisms able to produce 1,3-propanediol and optimization of medium constituents for Klebsiella pneumoniae AJ4
Microbial fermentation under anaerobic and microaerobic conditions has been used for the production of 1,3-propanediol (1,3-PD), a monomer used to produce polymers such as polytrimethyleneExpand
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Optimization of culture medium for enhanced production of exopolysaccharide from Aureobasidium pullulans
Polysaccharides produced by microorganisms are utilized for a variety of purposes, including the use in cosmetics and as food additives. More recently, polysaccharides have been exploited by theExpand
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Improved enantioselectivity of thermostable esterase from Archaeoglobus fulgidus toward (S)-ketoprofen ethyl ester by directed evolution and characterization of mutant esterases
Thermostable esterases have potential applications in various biotechnology industries because of their resistance to high temperature and organic solvents. In a previous study, we isolated anExpand
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Improving the enantioselectivity of an esterase toward (S)-ketoprofen ethyl ester through protein engineering
Abstract Esterases, enzymes that hydrolyze ester bonds, have been utilized to synthesize optically pure compounds like (S)-ketoprofen, which can be produced by the esterase-catalyzed hydrolysis ofExpand
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Optimization of alkaline pretreatment on corn stover for enhanced production of 1.3-propanediol and 2,3-butanediol by Klebsiella pneumoniae AJ4
Corn stover is one of the most promising lignocellulosic biomass that can be utilized for producing 1,3-propanediol and 2,3-butanediol. The pretreatment and enzymatic hydrolysis steps are essentialExpand
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Optimization of dilute sulfuric acid pretreatment of corn stover for enhanced xylose recovery and xylitol production
Pretreatment steps are necessary for the bioconversion of corn stover (CS) to xylitol. In order to optimize the pretreatment parameters, the sulfuric acid concentration, sulfuric acid residence time,Expand
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Strategies for increasing heterologous expression of a thermostable esterase from Archaeoglobus fulgidus in Escherichia coli.
Heterologous proteins expressed in bacteria are used for numerous biotechnological applications. Escherichia coli is the most commonly used host for heterologous protein expression because of itsExpand
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Crystal structure and characterization of esterase Est25 mutants reveal improved enantioselectivity toward (S)-ketoprofen ethyl ester
Esterases comprise a group of enzymes that catalyze the cleavage and synthesis of ester bonds. They are important in biotechnological applications owing to their enantioselectivity, regioselectivity,Expand
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