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A novel polypeptide, designated p18, was detected in a variety of hematopoietic cells by two-dimensional polyacrylamide gel electrophoresis. Quantitative analysis of p18 indicated its occurrence in a substantially greater amount in acute leukemia relative to nonleukemic cells. The increased amount of p18 in leukemia could not be explained on the basis of(More)
Two unrelated children, a boy 2 1/2 years old and a girl 4 years old, were affected with the cachectic dwarfism of Cockayne syndrome. Fibroblast cultures derived from these patients exhibited increased sensitivity to ultraviolet (UV) light, but not to x-irradiation, as measured by colony-forming ability. In both Cockayne fibroblast cultures, the rate of(More)
The cytotoxic action of physical and chemical agents on 10 skin fibroblast strains in culture derived from individuals with Cockayne's syndrome was measured in terms of colony-forming ability. As compared to fibroblasts from normal donors, all Cockayne cell strains tested exhibited a significantly increased sensitivity to UV light and a normal sensitivity(More)
Sixty-seven galactose negative (Gal minus) clones were established from survivors after treatment of Chinese hamster lung (V79) cells with 5-bromodeoxyuridine and black light. The mutational origin of these clonal isolates was inferred on the basis of the previously demonstrated mutagenic action of the combined treatment, persistence of the Gal minus(More)
Chemical induction of point mutations in mammalian tissue culture cells will make possible a study in these cells of genetic events at the molecular level similar to previous studies with microorganisms. Most reports on chemical induction of mutations in mammals describe studies of chromosome aberrations,' dominant lethals,2' 3 variegated-type position(More)