Eu. I. Golovanov

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The promoters on ColE1 plasmid DNA have been localized. Their position has been determined relative to the functional map of the plasmid. The direction of transcription from each promoter has been established. Superhelical ColE1 DNA was transcribed in vitro by Escherichia coli RNA polymerase. The resulting complexes of DNA with nascent RNA were treated with(More)
The fine structure of the melting curve for the linear colE1 DNA has been obtained. To find the ColE1 DNA regions corresponding to peaks in the melting curve's fine structure, we fixed the melted DNA regions with glyoxal /12/. Electron-microscopic denaturation maps were obtained for nine temperature points within the melting range. Thereby the whole process(More)
We have presented the method for recognition of structural domains of DNA. This method uses statistical description of coding and non-coding regions in the form of stationary or nonstationary Marcov chain, which was introduced in our previous papers. Calculation of the probability that the given fragment of the DNA appears part of the coding region, is the(More)
The problem of subclone identification for DNA fragments of a known nucleotide sequence has been considered. We suggest a strategy for rapid identification of a large number of subclones based on: (i) partial sequencing of the subclone DNA (single nucleotide track); (ii) representation of the result in the form of a numeric code showing the distribution of(More)
Analysis of the frequencies of occurrence of mono- and dinucleotides in sequenced E. coli DNA fragments was performed. The DNA sequences of total length 135 000 nucleotides were considered. It was demonstrated that the fragments of DNA which have different functional properties also have different parameters of neighbour nucleotides correlation. Moreover,(More)
A scheme of fast similarity search of nucleotide sequences is suggested based on sequence imaging, which results in chunks of information much less than original sequence but more specialized for comparison. Three methods were developed using three different imaging functions. The first is based on identity of local sites of up to twelve nucleotides, the(More)
Previously we presented some evidence for involvement of the imported tRNA1Lys in splicing the mitochondrial mosaic genes transcripts in yeast. The new data provided here together with our previous results show that tRNA1Lys/transcripts complementarity permits accurate prediction of intron-exon donor boundaries for at least 7 (probably all 9) Dujonian 1st(More)
We introduced non-stationary Marcov chains for statistical description of the DNA E. coli structural domains. The values of all needed parameters for those chains was determined by the preliminary statistical processing of a wide set of the E. coli coding regions. It was shown that non-stationary models predict frequencies of occurrences of various(More)
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