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Dual function of UPF3B in early and late translation termination
Nonsense‐mediated mRNA decay (NMD) is a cellular surveillance pathway that recognizes and degrades mRNAs with premature termination codons (PTCs). The mechanisms underlying translation terminationExpand
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Structure of a human cap-dependent 48S translation pre-initiation complex
Abstract Eukaryotic translation initiation is tightly regulated, requiring a set of conserved initiation factors (eIFs). Translation of a capped mRNA depends on the trimeric eIF4F complex and eIF4BExpand
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Targeted Nanopore Sequencing with Cas9 for studies of methylation, structural variants, and mutations
Nanopore sequencing technology can rapidly and directly interrogate native DNA molecules. Often we are interested only in interrogating specific areas at high depth, but conventional enrichmentExpand
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Targeted nanopore sequencing with Cas9-guided adaptor ligation
Despite recent improvements in sequencing methods, there remains a need for assays that provide high sequencing depth and comprehensive variant detection. Current methods 1 – 4 are limited by theExpand
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New insights into the interplay between the translation machinery and nonsense-mediated mRNA decay factors
Faulty mRNAs with a premature stop codon (PTC) are recognized and degraded by nonsense-mediated mRNA decay (NMD). Recognition of a nonsense mRNA depends on translation and on the presence ofExpand
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Analysis of short tandem repeat expansions and their methylation state with nanopore sequencing
Expansions of short tandem repeats are genetic variants that have been implicated in several neuropsychiatric and other disorders, but their assessment remains challenging with currentExpand
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Repeat expansion and methylation state analysis with nanopore sequencing
Expansions of short tandem repeats are genetic variants that have been implicated in neuropsychiatric and other disorders but their assessment remains challenging with current molecular methods.Expand
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A new link between translation termination and NMD complexes
Premature termination codons (PTCs) account for approximately one third of inherited and acquired diseases. A surveillance pathway called nonsense-mediated mRNA decay (NMD) detects and degradesExpand