Ethan Mirsky

Learn More
Microbial engineering often requires fine control over protein expression—for example, to connect genetic circuits or control flux through a metabolic pathway. To circumvent the need for trial and error optimization, we developed a predictive method for designing synthetic ribosome binding sites, enabling a rational control over the protein expression(More)
More significantly, the work is representative of an era when there was significant activity devoted to proposing new architectures for reconfigurable computing. VLSI technology was still simple enough that universities could contemplate and prototype significant designs with graduate student labor within the timeframe of a graduate degree. In this paper,(More)
The type III secretion system (T3SS) exports proteins from the cytoplasm, through both the inner and outer membranes, to the external environment. Here, a system is constructed to harness the T3SS encoded within Salmonella Pathogeneity Island 1 to export proteins of biotechnological interest. The system is composed of an operon containing the target protein(More)
Microbial engineering often requires fine control over protein expression; for example, to connect genetic circuits 1-7 or control flux through a metabolic pathway 8-13. We have developed a predictive design method for synthetic ribosome binding sites that enables the rational control of a protein's production rate on a proportional scale. Experimental(More)
Methyl halides are used as agricultural fumigants and are precursor molecules that can be catalytically converted to chemicals and fuels. Plants and microorganisms naturally produce methyl halides, but these organisms produce very low yields or are not amenable to industrial production. A single methyl halide transferase (MHT) enzyme transfers the methyl(More)
The records concerning 64 hips in 36 cerebral palsy patients treated with varus derotational osteotomy (VDO) alone (2 hips), VDO with muscle releases (adductors, iliopsoas, hamstrings; 46 hips), and VDO, soft-tissue release, and innominate bone osteotomy (16 hips) were reviewed. There were 23 quadriplegic and 13 diplegic patients. Average age at surgery was(More)
Gram-negative bacteria secrete proteins using a type III secretion system (T3SS), which functions as a needle-like molecular machine. The many proteins involved in T3SS construction are tightly regulated due to its role in pathogenesis and motility. Here, starting with the 35 kb Salmonella pathogenicity island 1 (SPI-1), we eliminated internal regulation(More)
MATRIX is a novel, coarse-grain, reconfigurable computing architecture which supports configurable instruction distribution. Device resources are allocated to controlling and describing the computation on a per task basis. Application-specific regularity allows us to compress the resources allocated to instruction control and distribution, in many(More)
The observations and experiments presented in this paper are concerned with the role of the protein component of ribonucleoprotein in synthesis of protein. This work shows that the nucleoprotein is part of the cell's equipment for protein synthesis and provides evidence that the protein component of nucleoprotein functions as precursor material for proteins(More)