Esther Kokenberg

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In an attempt to identify pharmacokinetic factors that determine the response of acute myeloid leukemia (AML) patients to induction chemotherapy, we determined the concentrations of daunorubicin (DNR) and the main metabolite daunorubicinol (DOL) in vivo and particularly evaluated the concentrations in blood and bone marrow nucleated cells. Cell measurements(More)
Twenty-one adult patients with acute myeloid leukemia (AML) were treated with the EORTC LAM-6 remission induction protocol [daunorubicin (DNR) (45 mg/m2, days 1-3), cytarabine (200 mg/m2, days 1-7) and vincristine (1 mg/m2, day 2)]. Pharmacokinetics of DNR were studied at day 1. The concentration of DNR and daunorubicinol were determined in plasma, in white(More)
Thirteen patients in complete remission from acute nonlymphoblastic leukaemia or in chronic phase of chronic myelocytic leukaemia were treated with total body irradiation, cyclophosphamide and allogeneic bone marrow transplantation (BMT). Ciclosporin (CS) was administered for the prevention and the treatment of Graft versus Host Disease. Blood(More)
Fluorescence distributions of thymocytes stained for Thy-1 as well as size measurements were used to discriminate between thymocyte subpopulations during regeneration of the thymus after irradiation and bone marrow transplantation. Subpopulations with "low" and "high" Thy-1 density of donor- and recipient-derived progeny were quantitated. They were(More)
Monitoring of daunorubicin (DNR) concentrations in leukemic cells in blood and bone marrow in vivo of patients with acute myeloid leukemia may yield insight into the interindividual variations of the clinical response to treatment. We evaluated the applicability of flow cytometry for measuring DNR uptake in direct comparison with high performance liquid(More)
The capability of nucleated blood cells and leukemic cells to transport daunomycin (DNR) to target tissues in the body was investigated in the rat. The in vivo distribution kinetics of DNR entrapped in leukemia cells (brown Norway acute myeloid leukemia, BNML) or in nucleated bone marrow cells, which had been exposed to DNR (0.2 mg/5 X 10(7) cells) in(More)
Critical technical parameters to establish a reliable method for quantifying the intracellular content of anthracyclines were evaluated in patients with acute myelocytic leukemia (AML); two methods were used for the isolation of leukocytes from the peripheral blood and two methods, for the extraction of daunorubicin (DNR), daunorubicinol (DOL), and(More)
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