Erwin Nijhuis

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PURPOSE The aim of the study was to investigate the molecular mechanisms involved in apoptosis of human promyelocytic cells (HL60) induced by hyperthermia and to compare this to radiation-induced apoptosis as a reference model. MATERIALS AND METHODS Apoptosis of HL60 cells was induced by heat-treatment (430C during 1 h) or by gamma-radiation (8 Gy) and(More)
Heat-induced apoptosis proceeds via mitochondria by permeabilization of the outer mitochondrial membrane (MOMP), resulting in the release of cytochrome c. This essential step is mediated by Bcl-2 family proteins, such as Bax. Recently, caspase-2 was assigned a prominent role in regulating Bax. Therefore, we studied the initiation of heat-induced apoptosis(More)
Cavitation bubble dynamics close to a rigid surface gives rise to a rapid and transient fluid flow. A single bubble is created with a laser pulse at different stand-off distances from the rigid surface, where the stand-off distance gamma is defined by gamma = h/R(max), with h being the initial distance and R(max) being the maximum bubble radius. When the(More)
PURPOSE The effect of heat treatment in combination with X-irradiation was examined with regard to expression of p53, a tumor suppressor gene product, and Hsp70, a heat-shock protein, in association with the occurrence of programmed cell death (apoptosis). MATERIALS AND METHODS Three hematopoietic cell lines (HSB2, HL60 and Kasumi-1), which differ in p53(More)
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