Spermatogenesis is the process by which spermatogonial stem cells divide and differentiate to produce sperm. In vitro sperm production has been difficult to achieve because of the lack of a culture system to maintain viable spermatogonia for long periods of time. Here we report the in vitro generation of spermatocytes and spermatids from… (More)
Differentiating male germ cells express a testis-specific form of cytochrome c (Cyt c(T)) that is distinct from the cytochrome c expressed in somatic cells (Cyt c(S)). To examine the role of Cyt c(T) in germ cells, we generated mice null for Cyt c(T). Homozygous Cyt c(T)(-/-) pups were statistically underrepresented (21%) but developed normally and were… (More)
Mouse testes contain a unique form of cytochrome c. As demonstrated by the indirect immunofluorescence technique, the testis-specific cytochrome c is detectable in the primary spermatocyte and in cell types comprising the later stages of spermatogenesis. Interstitial cells, Sertoli cells, and spermatogonia contain the somatic form of cytochrome c, as does… (More)
Computational methods were used to predict the amino acid sequences and gene locations for mammalian lactate dehydrogenase (LDH) genes and proteins using genome sequence databanks. Human LDHA, LDHC and LDH6A genes were located in tandem on chromosome 11, while LDH6B and LDH6C genes were on chromosomes 15 and 12, respectively. Opossum LDHC and LDH6B genes… (More)
The H-Y (male) antigen is expressed on 50 percent of eight-cell embryos (2 days old) of the mouse, strain C57BL/6. This work provides additional evidence that H-Y expression is not dependent on male differentiation.
Female mice of the C3H strain normally do not reject skin grafts from males of the same strain; however, 40 percent of splenectomized C3H female mice completely rejected C3H male skin grafts applied 2 weeks later. All splenectomized females showed at least transitory signs of graft rejection.
Clinical knowledge of subjective experience is considered essential for formulation of appropriate treatment expectations and goals. This study examined the relationship between self-reported depression and affective and cognitive correlates. Results indicated that girls reporting more depression had significantly earlier developmental levels of object… (More)
The ability of female mice to reject H-Y-incompatible, but otherwise histocompatible, male skin grafts differs greatly from strain to strain, as is illustrated particularly by the C57BL strain (B6 and other sublines), termed "H-Y rejector," because females invariably and promptly reject C57BL male skin, in comparison with the C3H strain, termed "H-Y… (More)