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Four RNA transcripts encoded by cauliflower mosaic virus DNA have been detected in the polyadenylated RNA from virus-infected turnip leaves. Two of these transcripts, the major 35S and the 8S species, have the same 5' termini, at nucleotide 7435. A viral DNA fragment encompassing this region directs transcription initiation at this point in vitro. The 5'(More)
The complete nucleotide sequence of carnation mottle genomic RNA (4003 nucleotides) is presented. The sequence was determined for cloned cDNA copies of viral RNA containing over 99% of the sequence and was completed by direct sequence analysis of RNA and cDNA transcripts. The sequence contains two long open reading frames which together can account for(More)
A sequence of 9703 nucleotides (nt) is reported for the genomic RNA of potato virus Y (Hungarian isolate, PVY-H), which causes necrotic rings around the buds on the tubers and mottling of leaves. The sequence contains one large open reading frame of 3061 amino acids (aa), a noncoding region of 189 nt at the 5' end and a 330-nt 3' nontranslated region. The(More)
Cucumber mosaic virus (CMV) and tomato aspermy virus (TAV) are closely related cucumoviruses. We have made pseudorecombinant viruses in which the RNAs 3 of these two viruses have been exchanged and recombinant viruses containing chimeric RNA 3 molecules, in which the coat proteins and the 3'-end regions of CMV and TAV have been exchanged, giving rise to(More)
Nicotiana benthamiana Domin. plants were transformed with the cytoplasmic inclusion protein (CI) gene of plum pox potyvirus (PPV) to investigate, whether this non-structural protein would be able to confer resistance. The CI protein is an RNA helicase, which contains a conserved nucleotide binding motif (NTBM) and plays an important role in viral(More)
A simple transformation procedure with a positive selection scheme using the expression of the isopentenyl transferase (ipt) gene of transfer DNA (T-DNA) 'shooter' mutants of Agrobacterium tumefaciens was elaborated. After comparing several 'shooter' mutants we found that particular strains frequently produced phenotypically normal shoots after(More)
The unique Ns isolate of Cucumber mosaic virus (CMV) induces necrotic lesions on several Nicotiana spp. in contrast to other strains that cause systemic mosaic on these plants. By using biologically active RNA transcripts from cDNAs of Ns-CMV and a reference subgroup I strain Rs-CMV, we confined the genetic determinant solely responsible for necrosis(More)
Coat proteins (CP) of five cucumovirus isolates, Cucumber mosaic virus (CMV) strains R, M and Trk7, Tomato aspermy virus (TAV) strain P and Peanut stunt virus (PSV) strain Er, were constructed by homology modelling. The X-ray structure of the Fny-CMV CP subunit B was used as a template. Models of cucumovirus CPs were built by the MODELLER program. Model(More)
Infectious hybrid virus was generated by replacing part of the coat protein gene of plum pox potyvirus with that of the zucchini yellow mosaic potyvirus. This viable hybrid contains 84.5% of zucchini yellow mosaic potyvirus coat protein gene while the rest of the sequence was derived from plum pox potyvirus. Changing the coat protein gene between these two(More)
The double-stranded DNA from the isolate D/H with an altered virulence of the cauliflower mosaic virus (CaMV) contains 8016 bp. The DNA is circular and possesses, like the DNA of most CaMV strains, three sequence interruptions. The comparison of its sequence with the previously published sequences of two other CaMV strains (Cabb-S and CM 1841) leads to the(More)