Ernesto San-Blas

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The life cycle of four Steinernema species was observed in 4 insect micro-insect host species (less than 5mm long). Several parameters were measured: sex ratio of invading nematodes, percentages of host infection and offspring production, penetration rate of infective juveniles per insect and number of new generation of infective juveniles. All parameters(More)
The use of Fourier transform mid-infrared spectroscopy with attenuated total reflection for characterizing entomopathogenic bacteria from genera Xenorhabdus and Photorhabdus is evaluated for the first time. The resulting spectra of Xenorhabdus poinarii and Photorhabdus luminiscens were compared with the spectrum of Escherichia coli samples. The absorption(More)
Entomopathogenic nematodes cannot be considered only as parasitic organisms. With dead Galleria mellonella larvae, we demonstrated that these nematodes use scavenging as an alternative survival strategy. We consider scavenging as the ability of entomopathogenic nematodes to penetrate, develop and produce offspring in insects which have been killed by causes(More)
Entomopathogenic nematodes complete their life cycles inside dead insects. The emergence of new infective juveniles from the cadaver has been attributed (but never demonstrated) to food depletion or to the accumulation of metabolites from the breakdown of the host's tissues. Here we give evidence that emergence is triggered by ammonia, a product of nematode(More)
During a survey in western Venezuela in 2011, three new populations of Heterorhabditis amazonensis (LPV081, LPV156, and LPV498) were isolated. Some differences were found in terms of morphometry compared with the original description; however, the distance from the anterior end to the excretory pore is the most variable character; significantly shorter in(More)
The life cycle of entomopathogenic nematodes (EPN) occurs inside an insect cadaver and an accumulation of ammonia initiates as a consequence of the nematodes defecation. This accumulation reduces the food resources quality and creates a detrimental environment for nematodes. When a given ammonia concentration is reached, the nematodes start their emergence(More)
Two populations (CS19 and CS20) of entomopathogenic nematodes were isolated from the soils of vegetable fields from Bijnor district, India. Based on morphological, morphometrical, and molecular studies, the nematodes were identified as Steinernema surkhetense. This work represents the first report of this species in India. The infective juveniles (IJs)(More)
Fungus gnats (FG) have been reported in Venezuelan greenhouses recently. Bradysia difformis Frey (Diptera, Mycetophilidae) was found attacking Daisy gerbera plants [Gerbera sp. L. (Asterales: Asteraceae)] and many other crops in the country causing serious damages. Heterorhabditis amazonensis Andaló et al. (Rhabditida: Heterorhabditidae), was used to assess(More)
A nematode from the genus Steinernema was isolated in Zulia state (North-western, Venezuela). Morphological, morphometric and molecular studies indicated that this nematode belongs to the "bicornutum" group and is described herein as Steinernema goweni n. sp. Steinernema goweni n. sp. is characterised by morphometrics of its infective juveniles, with body(More)
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