Ernest Hiebert

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ABSTRACT Tomato yellow leaf curl virus (TYLCV), a member of the genus Begomovirus (family Geminiviridae), causes severe losses in tomato production in the tropics and subtropics. In order to generate engineered resistance, eight different constructs of the TYLCV replication-associated protein (Rep) and C4 gene sequences were tested in transformed tomato(More)
The sequences of cDNA clones encoding most of the NIb protein, the coat protein and the 3' untranslated region of papaya ringspot virus (PRV) strains W and P have been determined. The open reading frame of P strain PRV was confirmed by amino acid analysis. Nucleotide sequence comparisons of these strains show that they share a 98.2% identity in their NIb(More)
A new geminivirus, tomato mottle virus (TMoV), affecting tomato production in Florida has been cloned and sequenced. Sequence analysis of the cloned replicative forms of TMoV revealed four potential coding regions for the A component [2601 nucleotides (nt)] and two for the B component (2541 nt). Comparisons of the nucleotide sequence of the TMoV genome with(More)
A caladium isolate of dasheen mosaic virus (DsMV-Ch) was cloned as cDNA from genomic RNA. The sequence of the 3′-terminal 3 158 nucleotides, which consisted of the 3′-terminus of the NIa gene, the NIb gene, the coat protein (CP) gene, and a 246-nucleotide non-coding region, was between 57–68% similar at the nucleotide level and 72–82% similar at the amino(More)
Tobacco plants were transformed with the movement protein (pathogenicity) gene (BC1) from tomato mottle geminivirus (TMoV), using Agrobacterium-mediated transformation. Different transgenic tobacco lines that expressed high levels of the BC1 protein had phenotypes ranging from plants with severe stunting and leaf mottling (resembling geminivirus symptoms)(More)
Squash mosaic virus, a member of the comovirus group, has a divided genome identified as middle-component RNA (M RNA; MW = 1.4 x 10(6)) and bottom-component RNA (B RNA; MW = 2 x 108). The isometric capsid of squash mosaic virus is composed of two distinct protein monomers with molecular weights of 22,000 (22k) and 42k. The isolated RNA components were(More)
The midguts of Bemisia tabaci and Trialeurodes abutilonea are looped so that the anterior and posterior extremities are in contact with each other. The basal lamina is breached at this point so that opposing basal epithelial membranes of either extremity are contiguous. On the anal side of the contact is a “filter-organ”, consisting of oesophageal,(More)
The translations of tobacco etch virus (TEV) RNA and pepper mottle virus (PeMV) in a rabbit reticulocyte lysate were analyzed to determine the effect of RNA quality on template activity and to identify all the products of the potyviral genome. The size distribution of the in vitro translation products, as analyzed by sodium dodecyl sulfate-polyacrylamide(More)
The nucleotide sequence of the 5'-terminal P1 coding region of an aphid-transmissible isolate of zucchini yellow mosaic virus (ZYMV; strain FL/AT), a mild isolate (strain MD) and a severe isolate (strain SV), all from Florida, were compared with two other ZYMV isolates. The ZYMV MD and SV isolates and an isolate from California (ZYMV CA) had 95-98% sequence(More)