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Although great progress has been made in the characterization of the off-target effects of engineered nucleases, sensitive and unbiased genome-wide methods for the detection of off-target cleavage events and potential collateral damage are still lacking. Here we describe a linear amplification-mediated modification of a previously published high-throughput,(More)
Targeting endogenous loci in live cells with nucleases designed to generate DNA double-stranded breaks (DSBs) at specific endogenous sequences without the need for substrate integration has been very useful for introducing targeted mutations and holds great promise for targeted gene therapy in humans 1–4. In this regard, the recently developed TALENs(More)
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