Erik J. De Vries

Learn More
The -amino acid ester hydrolase from Acetobacter turbidans ATCC 9325 is capable of hydrolyzing and synthesizing -lactam antibiotics, such as cephalexin and ampicillin. N-terminal amino acid sequencing of the purified -amino acid ester hydrolase allowed cloning and genetic characterization of the corresponding gene from an A. turbidans genomic library. The(More)
A new penicillin acylase was isolated by cloning and functional screening of DNA isolated from a sand soil enrichment culture. Sequence analysis of this enzyme, PAS2, revealed homology to a group of prominent penicillin G acylases, including the intensively studied enzyme of Escherichia coli ATCC 11105. Accordingly, PAS2 was found to be an Ntn-hydrolase(More)
Using direct and cell extraction-based (indirect) isolation methods, DNA was obtained from environmental samples with largely differing characteristics (loam soil, sand soil, sediment, activated sludge, and compost) and evaluated with respect to the comprised bacterial diversity and its suitability for expression cloning in Escherichia coli. Indirect DNA(More)
We study, in detail, the supersymmetric quantum mechanics of charge-(1, 1) monopoles in N = 2 supersymmetric Yang-Mills-Higgs theory with gauge group SU(3) spontaneously broken to U(1)×U(1). We use the moduli space approximation of the quantised dynamics, which can be expressed in two equivalent formalisms: either one describes quantum states by Dirac(More)
  • 1