Erick Barnes

Learn More
The ceca of newly hatched chicks obtained either from commercial hatcheries or from a carefully controlled experimental hatchery have been shown to contain high numbers of a variety of microorganisms. Fecal streptococci, clostridia, enterobacteria, pediococci, and occasionally Pseudomonas aeruginosa have all been isolated, but never lactobacilli. After the(More)
1. gamma-Aminobutyric acid (GABA) (10-500 microM) was applied to cultured chick cerebral neurons by pressure ejection, and the resulting currents (IGABA) were recorded using standard whole-cell voltage-clamp techniques. Plots of the peak IGABA as a function of membrane potential were nonlinear with an outwardly rectifying appearance. 2. IGABA decayed during(More)
The cerebellar granule cells of the spontaneous recessive mutant mouse strain, stargazer (stg/stg), fail to express brain-derived neurotrophic factor mRNA. This deficit is exclusive to these neurons and is believed to underlie the motor irregularities displayed by stg/stg, though the molecular basis for their phenotype has still to be resolved.(More)
The effect of chloride ion on the transport of [3H]dopamine into synaptic vesicles purified from rat striatum has been evaluated. The inclusion of 10 mM chloride ion in the incubation medium produced a 100% increase in temperature-sensitive [3H]dopamine uptake into synaptic vesicles from approximately 1800 pmol/mg to 3600 pmol/mg of protein. Half-maximal(More)
The effects of several cyclic nucleotide analogs and of phosphodiesterase inhibitors on the release of norepinephrine (NE) and dopamine-beta-hydroxylase activity (DBH) by electrical stimulation were studied in the isolated, perfused cat spleen. N-6-butyryl-3',5'-adenosine monophosphate (mbcAMP), 8-methylthio-3',5'-adenosine monophosphate,(More)
Thirty-two different types of anaerobic bacteria isolated from chickens have been tested for anti-salmonella activity in vitro. Under the conditions of the test only Bacteroides hypermegas and a Bifidobacterium sp. were shown to inhibit the salmonellas and this was attributed to the production of volatile fatty acids (VFA's) coupled with a low pH. When(More)
The effect of exogenous unsaturated fatty acids on the fatty acid composition of phospholipids of a mutant of E. coli has been examined. These exogenous acids serve as growth factors for an auxotroph requiring unsaturated fatty acids. When each member of a structurally homologous series of cis-unsaturated fatty acids serves as a growth factor, the(More)