Eric S Underbakke

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Fundamental to our understanding of biological systems is the ability to define interaction surfaces and conformational changes in dynamic protein complexes, yet this objective remains a challenge. Protein footprinting is a powerful solution to this problem. Methods such as hydrogen–deute-rium exchange, [1] limited proteolysis, [2] and radiolytic cleav-age(More)
Mammalian sGC is a heterodimer composed of a-and b-subunits (Figure 1) [1]. The C-terminus of each sub-unit contains a catalytic domain, and the active site is composed of residues from both subunits. Sequence analysis shows that each subunit also contains a well-defined PAS-like domain, and a predicted helical region. The N-termini of the a-and b-subunits(More)
Advances in genomic and proteomic methods provide the means to ascertain not only what receptors are present in a given cell but also how prevalent they are. While it is reasonable to assume that receptor concentration has a critical role in cell physiology, a new study published in this issue of The EMBO Journal suggests that the more relevant parameter(More)
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