Eric R Welsh

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Methods of genetic engineering were applied to the design and biosynthesis of three extracellular matrix protein analogues constructed from identical elastin- and fibronectin-derived repeating units but characterized by different molecular weights in the range of 14,000 to 59,000. Expression levels were enhanced by the serendipitous choice of an N-terminal(More)
This study examines the spreading and adhesion of human umbilical vein endothelial cells (HUVEC) on artificial extracellular matrix (aECM) proteins containing sequences derived from elastin and fibronectin. Three aECM variants were studied: aECM 1 contains lysine residues periodically spaced within the protein sequence and three repeats of the CS5 domain of(More)
The present investigation focuses on the synthesis and application of a cross-linking agent that is compatible with the solubility characteristics of chitosan. A water-soluble, blocked-diisocyanate was prepared as a bisulfite adduct to 1,6-hexamethylene diisocyanate, which proved to be stable for several weeks in aqueous, acidic chitosan solutions at room(More)
A water-soluble, blocked diisocyante was used to cross-link chitosan under various degrees of solvation, including hydration to form hydrogels. Thermal cross-linking of films cast from various amounts of organic cosolvents was found to increase with increased level of cosolvent up to a solvation level of 17% (w/w) and to be more efficient than for films(More)
Chitosan is an abundant naturally occurring biopolymer that is biocompatible, of low toxicity and is well suited as a carrier for a range of active agents. Restrictions imposed by this biopolymers unique solubility requirements make if difficult to process. One approach to an internal setting system is to employ the water-soluble blocked isocyanate(More)
beta-Glucuronidase from bovine liver is able to catalyze transfer of several carbohydrates to furfuryl alcohol, an acid-sensitive diene, with transfer yields as high as 84%. Carbohydrates that were transferred in yields of 30% or higher include gluco-, galacto-, xylo-, and fucopyranose. Small variations in the configuration of the substrate hydroxyls lead(More)
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