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Rapid Microsatellite Identification from Illumina Paired-End Genomic Sequencing in Two Birds and a Snake
It is shown that direct Illumina Seq-to-SSR can identify and characterize thousands of potentially amplifiable SSR loci for as little as $10 per sample – a fraction of the cost of 454 sequencing.
Comparative phylogeography of pitvipers suggests a consensus of ancient Middle American highland biogeography
Aim We used inferences of phylogenetic relationships and divergence times for three lineages of highland pitvipers to identify broad‐scale historical events that have shaped the evolutionary history…
Rapid identification of thousands of copperhead snake (Agkistrodon contortrix) microsatellite loci from modest amounts of 454 shotgun genome sequence
Results using high‐throughput sequencing to obtain a large number of microsatellite loci from the venomous snake Agkistrodon contortrix, the copperhead were rapid, cost‐effective and identified thousands of useful micros satellite loci in a previously unstudied species.
Complex evolution in the Neotropics: the origin and diversification of the widespread genus Leptodeira (Serpentes: Colubridae).
Cenozoic biogeography and evolution in direct-developing frogs of Central America (Leptodactylidae: Eleutherodactylus) as inferred from a phylogenetic analysis of nuclear and mitochondrial genes.
Phylogeographic and population genetic analyses reveal multiple species of Boa and independent origins of insular dwarfism.
The Burmese python genome reveals the molecular basis for extreme adaptation in snakes
- T. Castoe, A. D. de Koning, D. Pollock
- BiologyProceedings of the National Academy of Sciences
- 2 December 2013
The python and king cobra genomes are compared along with genomic samples from other snakes and transcriptome analysis is performed to gain insights into the extreme phenotypes of the python, finding rapid and massive transcriptional responses in multiple organ systems that occur on feeding and coordinate major changes in organ size and function.
Higher-level phylogeny of Asian and American coralsnakes, their placement within the Elapidae (Squamata), and the systematic affinities of the enigmatic Asian coralsnake Hemibungarus calligaster…
Despite long-held beliefs of systematic affinities based on morphology and colour pattern, the results suggest that Hemibungarus is not (phylogenetically) a coralsnake, but instead shares an exclusive common ancestor with Afro-Asian elapine genera (Elapsoidea, Dendroaspis and Ophiophagus).
Thousands of microsatellite loci from the venomous coralsnake Micrurus fulvius and variability of select loci across populations and related species
- T. Castoe, Jeffrey W. Streicher, D. Pollock
- Biology, Environmental ScienceMolecular ecology resources
- 1 November 2012
The feasibility of using 454 shotgun sequences for microsatellite marker development in coralsnakes was investigated and the repetitive landscape characteristics across multiple snakes were evaluated.
Discovery of Highly Divergent Repeat Landscapes in Snake Genomes Using High-Throughput Sequencing
The copperhead had 23-fold greater levels of TE-related transcripts than the python, suggesting the possibility that greater TE activity in the copperhead is ongoing, and it is therefore possible that TE activity played a role in the evolution of major adaptations in snakes.