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This paper presents a fully automated method for atlas-based whole-body segmentation in non-contrast-enhanced Micro-CT data of mice. The position and posture of mice in such studies may vary to a large extent, complicating data comparison in cross-sectional and follow-up studies. Moreover, Micro-CT typically yields only poor soft-tissue contrast for(More)
In cancer surgery, intra-operative assessment of the tumor-free margin, which is critical for the prognosis of the patient, relies on the visual appearance and palpation of the tumor. Optical imaging techniques provide real-time visualization of the tumor, warranting intra-operative image-guided surgery. Within this field, imaging in the near-infrared light(More)
In this paper, we propose a fully automated articulated registration approach for whole-body 3D data of mice. The method is based on a hierarchical anatomical model of the skeletal system where we specified position and degrees of freedom for each joint. Model fitting is performed by traversing a hierarchical part-tree, which enables a coarse-to-fine(More)
PURPOSE Using three publicly available small-animal atlases (Sprague-Dawley rat, MOBY, and Digimouse), we built three articulated atlases and present several applications in the scope of molecular imaging. PROCEDURES Major bones/bone groups were manually segmented for each atlas skeleton. Then, a kinematic model for each atlas was built: each joint(More)
  • Bang-Wen Xie, Isabel M. Mol, Stijn Keereweer, Ermond R. van Beek, Ivo Que, Thomas J. A. Snoeks +3 others
  • 2012
Bioluminescence imaging (BLI) has shown its appeal as a sensitive technique for in vivo whole body optical imaging. However, the development of injectable tumor-specific near-infrared fluorescent (NIRF) probes makes fluorescence imaging (FLI) a promising alternative to BLI in situations where BLI cannot be used or is unwanted (e.g., spontaneous transgenic(More)
BACKGROUND Evaluation of novel drugs for clinical development depends on screening technologies and informative preclinical models. Here we developed a multicolor bioluminescent imaging platform to simultaneously investigate transcription factor NF-κB signaling and apoptosis. METHODS The human breast cancer cell line (MDA-MB-231) was genetically modified(More)
PURPOSE Quantification of osteolysis is crucial for monitoring treatment effects in preclinical research and should be based on MicroCT data rather than conventional 2D radiographs to obtain optimal accuracy. However, data assessment is greatly complicated in the case of 3D data. This paper presents an automated method to follow osteolytic lesions(More)
Fluorescence and bioluminescence imaging have different advantages and disadvantages depending on the application. Bioluminescence imaging is now the most sensitive optical technique for tracking cells, promoter activity studies, or for longitudinal in vivo preclinical studies. Far-red and near-infrared fluorescence imaging have the advantage of being(More)
  • Karien E. de Rooij, Martijn van der Velde, Edwin de Wilt, Martine M. L. Deckers, Martineke Bezemer, Jan H. Waarsing +4 others
  • 2015
Osteocytes are the predominant cells in bone, where they form a cellular network and display important functions in bone homeostasis, phosphate metabolism and mechanical transduction. Several proteins strongly expressed by osteocytes are involved in these processes, e.g., sclerostin, DMP-1, PHEX, FGF23 and MEPE, while others are upregulated during(More)
This paper discusses a number of image analysis challenges emerging from longitudinal small animal molecular imaging studies. Three steps towards a quantitative 3D analysis of follow-up small animal imaging are presented: whole-body articulated registration, change visualization in follow-up data and fusion of optical and 3D structural imaging data. Several(More)
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