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Bell pepper endornavirus: molecular and biological properties, and occurrence in the genus Capsicum.
Bell peppers (Capsicum annuum) harbour a large dsRNA virus. The linear genome (14.7 kbp) of two isolates from Japanese and USA bell pepper cultivars were completely sequenced and compared. TheyExpand
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Molecular characterization of two evolutionarily distinct endornaviruses co-infecting common bean (Phaseolus vulgaris).
Two high-molecular-mass dsRNAs of approximately 14 and 15 kbp were isolated from the common bean (Phaseolus vulgaris) cultivar Black Turtle Soup. These dsRNAs did not appear to cause obvious diseaseExpand
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Knock-down of OsDCL2 in rice negatively affects maintenance of the endogenous dsRNA virus, Oryza sativa endornavirus.
An endogenous double-stranded RNA (dsRNA), which has recently been recognized as the dsRNA virus Oryza sativa endornavirus (OsEV), is found in many strains of cultivated rice (Oryza sativa). SmallExpand
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A simple and rapid method to purify viral dsRNA from plant and fungal tissue
The occurrence of high-molecular-weight double-stranded RNA (dsRNA) is a hallmark of RNA virus infection in plants and fungi; thus, dsRNA profiling is an attractive tool for preliminary diagnosis orExpand
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An Arabidopsis RNase III-like protein, AtRTL2, cleaves double-stranded RNA in vitro
Class 1 ribonuclease III (RNase III), found in bacteria and yeast, is involved in processing functional RNA molecules such as ribosomal RNAs (rRNAs). However, in Arabidopsis thaliana, the lack of anExpand
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A new endornavirus species infecting Malabar spinach (Basellaalba L.)
Abstract A putative new endornavirus was isolated from Malabar spinach (Basella alba). The viral dsRNA consisted of 14,027 nt with a single ORF that coded for a polyprotein of 4,508 aa. The genomeExpand
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Molecular and biological properties of an endornavirus infecting winged bean (Psophocarpus tetragonolobus)
A double-stranded RNA (dsRNA) of approximately 15 kbp was isolated from asymptomatic winged bean (Psophocarpus tetragonolobus) plants. The size of the dsRNA, together with results of RT-PCR testing,Expand
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Detection of long and short double-stranded RNAs.
In RNA interference (RNAi), long double-stranded RNAs (dsRNAs) of more than 100 nucleotides (nt) are diced into short dsRNAs (small interfering RNAs, siRNAs) of about 21-24 nt, the guide strand ofExpand
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