Emmanuelle Helloin

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The natural protective system of eggs relies on egg yolk immunoglobulins and on antimicrobial proteins/peptides mainly concentrated in the egg white. There is much evidence concerning the specific stimulation of immunoglobulins by antigens but to date, the influence of the hen milieu on the regulation of the egg innate molecular immunity has not been(More)
The proteomes of Listeria monocytogenes expressed in suspension and biofilm state, in the presence and absence of a carbon source, were analysed by two-dimensional electrophoresis with the help of computer software. The up-regulated proteins in each case were identified by peptide sequencing using electrospray ionisation tandem mass spectrometry and a(More)
Ovalbumin family contains three proteins with high sequence similarity: ovalbumin, ovalbumin-related protein Y (OVAY), and ovalbumin-related protein X (OVAX). Ovalbumin is the major egg white protein with still undefined function, whereas the biological activity of OVAX and OVAY has not yet been explored. Similar to ovalbumin and OVAY, OVAX belongs to the(More)
Sophie Réhault-Godbert, Valérie Labas, Emmanuelle Helloin, Virginie Hervé-Grépinet , Cindy Slugocki, Magali Berges, Marie-Christine Bourin**, Aurélien Brionne, Jean-Claude Poirier, Joël Gautron, Franck Coste, and Yves Nys From the Institut National de la Recherche Agronomique, UR83 Recherches Avicoles, Fonction et Régulation des Protéines de l’Œuf, F-37380(More)
Egg defence against bacterial contamination relies on immunoglobulins (IgY) concentrated in the yolk and antimicrobial peptides/proteins predominantly localized in the egg white (EW). Hens contaminated with pathogenic microorganisms export specific IgYs to the egg (adaptative immunity). No evidence of such regulation has been reported for the antimicrobial(More)
An aqueous marine-sulfated polysaccharide (MSP) extract, prepared from the green macroalga Ulva armoricana, was tested as an antibacterial compound against 42 bacterial strains and isolates found in livestock animals. Both Gram-positive and Gram-negative bacteria growths were affected. The most susceptible pathogens were Pasteurella multocida, Mannheimia(More)
Escherichia coli can cause extraintestinal infections in humans and animals. The hlyF gene is epidemiologically associated with virulent strains of avian pathogenic E. coli and human neonatal meningitis-associated E. coli. We demonstrated that culture supernatants of E. coli expressing HlyF induced autophagy in eukaryotic cells. This phenotype coincided(More)
Egg white is an important contributor to the protection of eggs against bacterial contaminations during the first half of incubation (day zero to 12), prior to the egg white transfer into the amniotic fluid to be orally absorbed by the embryo. This protective system relies on an arsenal of antimicrobial proteins and on intrinsic physicochemical properties(More)
Cellulitis due to Escherichia coli is rare and usually secondary to a cutaneous portal of entry. Skin and soft tissue infections (SSTI) secondary to E. coli bacteraemia have been reported exclusively in immunodeficient patients. Here, we report two cases of serious cellulitis secondary to E. coli bacteraemia in patients with haematological malignancies.(More)
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