Emmanuel O Anosike

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The activities of human erythrocyte glutathione-S-transferase (EC obtained from 53 subjects of both sexes aged between 5 and 25 years of HbAA (20), HbAS (13) and HbSS (20) were determined. The results, expressed in IU/g Hb, obtained for the various genotypes were: HbSS = 14.6 +/- 3.4; HbAS = 4.7 +/- 1.1; HbAA = 2.7 +/- 0.8. The observed(More)
1. Some effects of anions on the rates of phosphoarginine synthesis by monomeric (lobster) and by dimeric (Holothuria forskali) arginine kinases are reported. 2. As with creatine kinase, acetate ions activate both enzymes: Cl- was also found to activate both although this was an inhibitor of creatine kinase. 3. NO3- inhibits the lobster enzyme. Inhibition(More)
The in vitro effects of aqueous extracts of Zanthoxylum macrophylla roots on the activities of three ATPases (Na, K; Ca; and Mg-ATPases) were investigated in membrane preparations from human erythrocytes of HbAA, HbAS and HbSS bloods. The activities of these membrane-bound enzymes were determined in the presence of varying concentrations of the plant(More)
Rhodanese (EC has been partially purified from guinea pig and rat kidneys. The specific activities of the preparations were 1.23 and 0.44 mumol of product formed per minute per milligramme of enzyme protein. The guinea pig kidney enzyme had Km values of 14.2 and 31.5 mmol/l for cyanide and thiosulphate, respectively, while for the rat kidney(More)
Whole blood cyanide levels in a human population sample of volunteers known to each gari-based meals at least once a day were determined. The most widely eaten cassava product in the area of research is gari. The mean blood cyanide obtained for healthy normal volunteers was 0.294 +/- 0.09 mumol/L while the value for sicklers was 0.398 +/- 0.008 mumol/L,(More)
1. The nature of arginine binding to lobster arginine kinase and the extent of its possible involvement with the ;essential' thiol group of the enzyme has been investigated with some inhibitory analogues of arginine. 2. Most of the analogues inhibit competitively, although mixed inhibition may occur if the alpha-carboxy group or alpha-amino group is absent.(More)
Reports on the influence of fructose on blood alcohol clearance have not always been consistent. Notwithstanding, information concerning the Nigerian population is yet to be documented. In this present study, ten consenting adults in apparent sound health, and who did not have any traceable history of alcohol or drug abuse were selected. The subjects were(More)
1. A purification procedure for the dimeric arginine kinase of the sea cucumber Holothuria forskali is described. 2. The enzyme has a mean molecular weight of 77250 and is composed of two equal, dissociable subunits. 3. It also shows co-operativity between substrate binding at one catalytic site to a much greater extent than the nomomeric lobster arginine(More)