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This study describes the biodegradation of phenanthrene in aqueous media in the presence and in the absence of a surfactant, Brij 30. Biodegradations were performed using either Pseudomonas putida DSMZ 8368 or a bacterial consortium Pyr01 isolated from one PAHs-polluted site. P. putida degraded phenanthrene to form 1-hydroxy-2-naphthoic acid (1H2Na) as the(More)
Tissue engineering may constitute a promising alternative to current strategies in ligament repair, providing that suitable scaffolds and culture conditions are proposed. The objective of the present contribution is to present the design and instrumentation of a novel 168 multi-chamber tension-torsion bioreactor dedicated to ligament tissue engineering. A(More)
Background During the production of any recombinant proteins, an evaluation of the product quality is crucial. Proteolytic events may occur during the process and could influence the product quality. Indeed, proteolysis is an unpredictable process and relatively little is know regarding the proteolytic enzymes produced and released by mammalian cells. In(More)
to develop a new strategy combining near-infrared (NIR) and dielectric spectroscopies for real-time monitoring and in-depth characterizing populations of Chinese hamster ovary cells throughout cultures performed in bioreactors. Spectral data processing was based on off-line analyses of the cells, including trypan blue exclusion method, and lactate(More)
To design novel 3D in vitro co-culture models based on the RGD-peptide-induced cell self-assembly technique. Multicellular spheroids from M-3 murine melanoma cells and L-929 murine fibroblasts were obtained directly from monolayer culture by addition of culture medium containing cyclic RGD-peptide. To reach reproducible architecture of co-culture spheroids,(More)
Background Expansion of mesenchymal stromal cells (MSC) is one of the key steps for their use in tissue engineering or cell therapies. To increase cells expansion yields, two milestones have to be achieved that will allow a wider MSC therapeutic use, namely an optimal serum-free medium and a process intensification via 3D suspension culture on microcarriers(More)
Background In the biopharmaceutical industry, the control of glyco-sylation to satisfy the quality consistency of recombinant proteins produced during a process has become an important issue. Indeed, the glycosylation pattern of recombinant proteins could be influenced by different factors including the cell line used, environmental factors such as(More)
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