Else-Maj Suolinna

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Certain bioflavonoids inhibit the glycolysis of variety of tumor cells by interfering with the generation of adenosine diphosphate and inorganic phosphate which are required for glycolysis. Tetra- and pentahydroxy flavones with hydroxyl groups as 3, 3', 4', 5, and 7 (e.g., quercetin) are the most active. They inhibit the activity of isolated(More)
In order to further establish optimal and reproducible conditions for the use of primary hepatocyte cultures in studies of drug metabolism, the effect of culture age on the basal and induced activities of ethoxycoumarin O-deethylase (ECDE), UDP-glucuronyltransferase (GT) [methylumbelliferone (MU) and p-nitrophenol (pNP) as substrates] and sulfotransferase(More)
The metabolism and covalent binding of the environmen tal carcinogen benzo(a)pyrene, a constituent of cigarette smoke, has been determined in isolated perfused lungs of shamand cigarette smoke-exposed and 3-methylcholanthrene-pretreated male Wistar rats. Rats were exposed to cigarette smoke for 1 hr daily for either 1 or 10 days. Benzo(a)pyrene (2 nmoles)(More)
The activities of hexokinase and glucose-6-phosphatase, as well as the in vivo metabolic products of 2-[18F]fluoro-2-deoxyglucose ([18F]FDG) (45 min after an i.v. injection), were determined from several tissues of Rous sarcoma implanted rats. The HK/G-6-Pase ratio was found to be high in brain and tumor, and low in liver with intermediate values for kidney(More)
Isolated liver cells are currently used in research on hepatic function and metabolism, e.g., in studies on the synthesis of plasma proteins, control of gluconeogenesis and metabolism of glycogen. In addition they are becoming an important tool in investigating the metabolism and action of xenobiotics, where they are used for studying basic metabolic(More)
Periportal hepatocytes isolated by digitonin/collagenase perfusion produced urea faster than did similarly prepared perivenous hepatocytes, in both the presence and the absence of amino acids and various urea precursors. There was no difference between the two cell types in rates of intracellular proteolysis. The initial difference in urea synthesis(More)