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Non-melanoma skin cancer includes basal cell carcinoma (BCC), squamous cell carcinoma (SCC) and Bowen's disease. The differential diagnosis of these lesions is sometimes difficult and relies on the histopathological examination of surgical specimens. However, a precise differential diagnosis is crucial for an accurate therapy and thus better patient care.(More)
FTIR spectral imaging was applied on formalin-fixed paraffin-embedded biopsies from colon and skin cancerous lesions. These samples were deposited onto different substrates (zinc selenide and calcium fluoride respectively) and embedded using two types of paraffin. Formalin fixation followed by paraffin embedding is the gold standard in tissue storage. It(More)
This study aims to develop IR imaging of tumor tissues for generating an automated IR-based histology. Formalin-fixed paraffin-embedded xenografts of human colon carcinomas were analyzed. Chemometric and statistical multivariate treatments of spectral data permitted to probe the intrinsic chemical composition of tissues, directly from paraffinized sections(More)
Mid-IR spectral imaging is an efficient method to analyze biological samples. Several research studies showed its potential to diagnose cancerous tissues. However, some limitations appear when formalin-fixed paraffin-embedded tissues are studied due to the intense IR contribution of paraffin, unless to perform a time-consuming and aggressive chemical(More)
This study aims to develop a new FT-IR spectral imaging of tumoral tissue permitting a better characterization of tumor heterogeneity and tumor/surrounding tissue interface. Infrared (IR) data were acquired on 13 biopsies of paraffin-embedded human skin carcinomas. Our approach relies on an innovative fuzzy C-means (FCM)-based clustering algorithm, allowing(More)
BACKGROUND The diagnosis of malignant melanoma is based upon the histological evaluation of the lesion. As such, the morphological interpretation relies on the expertise of a dermatopathologist. Infrared microimaging is emerging as a new powerful tool to investigate tissue biochemistry. Infrared spectra probe the biochemical constitution of the sample and(More)
Basal cell carcinoma (BCC) can sometimes lead, through a possible invasion of the dermis and the subcutaneous tissue, to serious local damage to the patient. Several histological types of BCC are reported, among them, the superficial, nodular and infiltrative forms. This study reports the use of polarized Raman microspectroscopy on the nodular and(More)
Polarized Raman microspectroscopy can provide precious information regarding the orientation and ordering of the molecules in a sample without staining or particular preparation. This technique is used for the first time on a human skin section to probe the molecular modifications of the surrounding dermis in superficial basal cell carcinoma. Spectra using(More)
Mid-Infrared (IR) micro-spectral imaging is an efficient method to analyze molecular composition of biomedical samples. In clinical oncology, this non-invasive technique is generally used on frozen biopsies to localize and diagnose cancerous tissues in their early stages. However, samples are usually fixed in paraffin in order to be preserved from decay,(More)
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