Elmas Gök

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In this paper, we reported the syntheses and investigation of the modes of binding to DNA of the two new ethidium derivatives containing benzoyl and phenylacetyl groups of both amines at 3-and 8- positions. The interactions between calf thymus DNA (ct-DNA) and the two derivatives, 3,8-dibenzoylamino-5-ethyl-6-phenylphenantridinium cloride (E2) and(More)
The interaction between thyroxine hormone and 7 hydroxycoumarin (7HC) was investigated using fluorescence quenching method. The experimental results showed that thyroxine could quench the fluorescence of 7HC by forming the 7HC–thyroxine complex with static quenching. The apparent binding constants (K) between 7HC and thyroxine were determined to be 1.51 ×(More)
The determination of the fluorophore to the protein molar ratio has been studied using fluorescence spectroscopy. The tyrosine fluorescence is measured from insulin (Ins) solutions at wavelengths λex/λem = 276/300 nm and from fluorescein isothiocyanate (FITC) solutions at λex/λem = 494/518 nm. Series of solutions prepared from insulin and FITC are tested(More)
The binding interaction of lysozyme and umbelliferone (7hydroxcoumarin, 7HC) was investigated by UV–vis absorption and fluorescence quenching. It was obtained from fluorescence spectra that the fluorescence quenching of lysozyme by 7HC was probably a result of the formation of lysozyme-7HC complex and binding parameters were determined according to the(More)
We describe a method for the determination of thyroxine (Thy) using its quenching effect on Eu(PDA)3 3- tris complex fluorescence. The relative fluorescence intensities are measured at fixed λexc = 282 nm, λem = 615 nm by titrating the metal complex with Thy in piperazine buffer solution at pH 6.5. Data indicated an associative type of reaction of two(More)
Hydrogen peroxide (H2O2) plays an important role in natural water samples. In this study H2O2 concentrations were quantified in different water samples by chemiluminescence detection. This method was chosen because of its high sensitivity and suitability for determining low concentrations of H2O2. H2O2 is introduced to the oxidation reaction of alkaline(More)
A series of ionogenic poly(HEMA) membranes which were prepared by bulk copolymerization of 2-hydroxyethylmethacrylate (HEMA) and anionic or cationic comonomers, acrylic acid (AA), and dimethylaminoethylmethacrylate (DMAEMA), were characterized by equilibrium swelling measurements, surface free energies, and protein adsorption studies. It was found that(More)
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