Ellen Pugh

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The molecular types of lecithin in beef heart lipids were investigated by a combination of mild hydrolytic procedures and silicic acid chromatography. The major species of sn-glycero-3-phosphorylcholine (GPC) were found to be: diacyl-GPC (57%), alk-l-enyl, acyl-GPC (39%), alkyl acyl-GPC (3%), and dialkyl-GPC ( less than 1%). No di-alk-l-enyl-GPC or(More)
Although the membrane lipids of extremely halophilic archaebacteria are exclusively derived from diphytanylglycerol diether, which is non-acylated, small amounts of fatty acids have been detected in these organisms. These fatty acids are formed by the action of a fatty acid synthase (FAS), shown to be present in the extreme halophile Halobacterium(More)
A microsomal enzyme system from rat liver was shown to catalyze desaturation, in presence of reduced pyridine nucleotides and oxygen, of 1-acyl-2-[14C]eicosatrienoyl-sn-glycero-3-phosphorylcholine to 1-acyl-2-[14C]arachidonoyl-sn-glycerophosphorylcholine. This desaturation was linear with time and proportional to microsomal protein concentration, and(More)
Phospholipid desaturase activity of rat liver microsomes can be varied by dietary manipulation: rats starved and refed a "fat-free" diet have two to three times the activity of normal controls whereas starved rats have no detectable activity. These changes were accompanied by changes in fatty acid composition of the microsomal phospholipids, resulting in a(More)
Several characteristics of the microsomal phospholipid desaturase of Candida lipolytica are described. The phospholipid desaturase reaction required molecular oxygen and reduced pyridine nucleotides as essential cofactors and was inhibited by cyanide but not by carbonmonoxide, indicating that it required cytochrome b5. Desaturation of both(More)