Ellen B. Peffley

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Regenerating calluses of Allium fistulosum × A. cepa interspecific F1 hybrids were treated in vitro with colchicine. A factorial experiment was designed to test the effects of colchicine concentration and time on the recovery of tetraploid plants from in vitro-colchicine-treated calluses. Shoot production of regenerating calluses following in vitro(More)
A biotin- and fluorescein-labelled probe of Helianthus argophyllus has been used to map specific repeated rDNA sequences by in situ hybridization on mitotic chromosomes of Alliwn cepa, Allium fistulosum, a diploid interspecific (Allium fistulosum x Allium cepa) F1 hybrid, and a triploid interspecific (2 x = A. cepa, 1 x = A. fistulosum) shallot.(More)
Meiotic pairing in an interspecific triploid of Allium cepa and A. fistulosum, ‘Delta Giant’, exhibits preferential pairing between the two A. cepa genomes, leaving the A. fistulosum genome as univalents. Multivalent pairing involving A. fistulosum chromosomes occurs at a low level, allowing for recombination between the genomes. Ten trisomies were(More)
Procedures were developed for disinfestation of non-dormant basal plate tissue excised from field grown basal plate tissue of diploid Allium fistulosum × A. cepa F1 hybrid onions. Contamination levels varied with the season and vegetative development of plant material. Callus initiated from basal plate tissue and immature inflorescences of the F1 hybrids(More)
Cytological analysis of (Allium cepa L.×Allium fistulosum L.)×A. cepa L. F1BC3 plants revealed most plants were diploid with 16 chromosomes. Karyotypes of these plants showed recombinant chromosomes. Fluorescence and genomic in situ hybridization patterns of interspecific F1 hybrid and F1BC3 plants revealed A. fistulosum chromosomes or chromosomal segments.(More)
Using divergent Giemsa staining protocols designed for other plant species, G-banding was visualized in the chromosomes of Allium cepa L., A. fistulosum L., and their interspecific (A. fistulosum x A. cepa) hybrid. This is the first demonstration of G-banding of Allium chromosomes. Differences and similarities with other Giemsa banding patterns in Allium(More)
Allium fistulosum possesses a number of traits which would be desirable in A. cepa. Thus far, no commercial A. cepa cultivars have been released which harbor Allium fistulosum traits. F1BC3 populations were generated for this study by backcrossing A. cepa to A. cepa×A. fistulosum hybrids. The F1BC3 plants were evaluated for plant morphology, floral(More)
A diploid Allium cepa plant was recovered from the backcross of an interspecific triploid (2 x A. cepa + 1 x A. fistulosum) to an A. cepa diploid which exhibited both A. cepa and A. fistulosum Adh-1 alleles. Cytogenetic analyses revealed a recombinant sub-telocentric chromosome. The ADH-1 locus is believed to be on the long arm of the sub-telocentric A.(More)
Elevated atmospheric CO2 concentrations ([CO2]) have been hypothesized to increase photosynthesis rates and volatile organic compound (VOC) emissions; however, field measurements from a select group of conifer and angiosperm trees have shown that VOC emissions are in fact not affected or reduced by elevated CO2 levels. To broaden the understanding of how(More)
Conflicting theories of inheritance of branching in guar [Cyamopsis tetragonoloba (L.) Taub.] have been reported. In this study, two lines differing in leaf surface and growth habit were used to make reciprocal crosses and form progeny populations in order to study how basal branching is inherited. Parents in the reciprocal crosses were PI 217923, which is(More)