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A review of modern works on the creation of biotechnological processes using cells immobilized on different carriers is presented. General material requirements are given for for immobilization mainly performed by absorption and mechanical fixation methods. The results of studies on cell immobilization are considered, and an analysis of the materials and… (More)
A review of current work in biodiesel production via enzymatic catalysis has been done. The parameters of the process as determined by laboratories are represented and analyzed. The main factors affecting interesterification are considered. The major types of oils and alcohols used in biodiesel synthesis are listed. The means of lipase enzyme… (More)
The Yarrowia lipolytica lipase Lip2p was displayed on the yeast cell surface via N-terminal fusion variant using cell wall protein YlPir1p. The hydrolytic activity of the lipase displayed on Y. lipolytica cells reached 11,900 U/g of dry weight. However, leakage of enzyme from the cell wall was observed. The calculated number of recombinant enzyme displayed… (More)
An analytical review of the current data on the microbial synthesis of biodiesel and prospects of its usage is presented. The technological, biochemical, and genetic aspects of biodiesel production using microorganisms are discussed.
The oleaginous yeast Yarrowia lipolytica is a convenient model for investigating lipid biosynthesis and for engineering high lipid accumulated strains. In this organism, the pentose phosphate pathway is the major source of NADPH for lipid biosynthesis. Thus, we over-expressed gene encoding NADP+-dependent glucose-6-phosphate dehydrogenase (ZWF1) in a strain… (More)