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Mutations in whole organisms are powerful ways of interrogating gene function in a realistic context. We describe a program, the Sanger Institute Mouse Genetics Project, that provides a step toward the aim of knocking out all genes and screening each line for a broad range of traits. We found that hitherto unpublished genes were as likely to reveal(More)
While there is substantial evidence that the food intake of laboratory animals in suppressed following administration of opiate antagonists, there is less known about the effects of opiate antagonists on human feeding. This study was undertaken to examine the effect of the relatively specific opiate antagonist, naloxone, on the food intake of normal human(More)
The CRISPR/Cas9 system has rapidly advanced targeted genome editing technologies. However, its efficiency in targeting with constructs in mouse zygotes via homology directed repair (HDR) remains low. Here, we systematically explored optimal parameters for targeting constructs in mouse zygotes via HDR using mouse embryonic stem cells as a model system. We(More)
The effect of dexamphetamine (d-Amp) and placebo on visual analogue scale (VAS) ratings of hunger, arousal and mood in nine male volunteers was observed. d-Amp (10 mg) significantly depressed hunger ratings but did not significantly affect arousal and mood ratings. d-Amp (20 mg) had a significant effect on all three ratings. There was a difference in the(More)
The interaction between the receptor antagonist thymoxamine (THYM), propranolol (PPL) and metergoline (MTG) with dexamphetamine (d-Amp)-induced anorexia was examined in a series of studies in normal female volunteers. Visual analogue scale (VAS) ratings of hunger were made and food intake was measured using an automated solid food dispenser (AFD). d-Amp (10(More)
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