Elizabeth S. Fleming

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After fertilization, maternal factors direct development and trigger zygotic genome activation (ZGA) at the maternal-to-zygotic transition (MZT). In zebrafish, ZGA is required for gastrulation and clearance of maternal messenger RNAs, which is in part regulated by the conserved microRNA miR-430. However, the factors that activate the zygotic program in(More)
BACKGROUND MicroRNAs (miRNAs) are small non-coding RNAs (18-24 nucleotides) that have recently been shown to regulate gene expression during cancer progression. Dicer, a central enzyme in the multi-component miRNA biogenesis pathway, is involved in cutting precursor miRNAs to functionally mature forms. Emerging evidence shows that Dicer expression is(More)
A major feature of epithelial cell polarity is regulated positioning of the mitotic spindle within the cell. Spindles in cells of symmetrically expanding tissues are predicted to align parallel to the tissue plane. Direct measurement of this alignment has been difficult in mammalian tissues. Here, we analyzed the position of spindles in intact mouse(More)
The full repertoire of human microRNAs (miRNAs) that could distinguish common (benign) nevi from cutaneous (malignant) melanomas remains to be established. In an effort to gain further insight into the role of miRNAs in melanoma, we applied Illumina next-generation sequencing (NGS) platform to carry out an in-depth analysis of miRNA transcriptome in(More)
Orientation of mitotic spindles plays an integral role in determining the relative positions of daughter cells in a tissue. LKB1 is a tumor suppressor that controls cell polarity, metabolism, and microtubule stability. Here, we show that germline LKB1 mutation in mice impairs spindle orientation in cells of the upper gastrointestinal tract and causes(More)
The Traffic alert and Collision Avoidance System (TCAS) is often framed as separate to-or opposing-air traffic control, i.e. as a backup, redundant system that invokes resolution advisories (RAs) when all other methods of separation assurance fail. A flight simulator experiment examined pilot interaction with TCAS in the context of a full air traffic(More)
Carlson's developmental theory of self-concept provides a theoretical explanation for equivalent levels of self-esteem among both sexes, despite sex differences in self-concept. The present study tests the applicability of Carlson's theory for a sample of gifted and talented female adolescents by examining three dimensions of possible self-esteem(More)
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