Elizabeth M. V. Jones-Villeneuve

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Murine embryonal carcinoma cells can differentiate into a varied spectrum of cell types. We observed the abundant and precocious development of neuronlike cells when embryonal carcinoma cells of various pluripotent lines were aggregated and cultured in the presence of nontoxic concentrations of retinoic acid. Neuronlike cells were also formed in retinoic(More)
Pluripotent murine embryonal carcinoma cells can differentiate in culture into many tissue types similar to those normally found in early embryos and may be useful in investigating some developmental events. Central to our understanding of embryonic development are explanations of cellular determination, that is, the commitment of early embryonic cells to(More)
We have previously shown that the P19 line of embryonal carcinoma cells develops into neurons, astroglia, and fibroblasts after aggregation and exposure to retinoic acid. The neurons were initially identified by their morphology and by the presence of neurofilaments within their cytoplasm. We have more fully documented the neuronal nature of these cells by(More)
Dexter has described a culture system in which pluripotent stem cells (CFUs) are maintained for several months on an adherent layer derived from bone marrow. The population of cells floating in the supernatant medium contains stem cells and other cells at later stages of myeloid differentiation. Mature lymphocytes have not been observed in these cultures.(More)
Lone-term marrow cultures, established according to the technique described by Dexter, contain a colony-forming cell (CFU-T) that produces Thy 1-positive progeny after stimulation with PHA-induced conditioned medium from human leukocytes. When 10(-7) M hydrocortisone is added to the medium for the long-term cultures CFU-T can be maintained in vitro for at(More)
Approximately 2,000 embryogenic uninuclear microspores of rapeseed (Brassica napus) cv. Topas were intranuclearly injected with a chimaeric β-glucuronidase (Escherichia coli Uid A) gene. Stable integration had not occurred among 55 plants that were regenerated. Coinjection of the dye Lucifer Yellow and detection of injected DNA by the polymerase chain(More)
Immunofluorescence staining with antibodies to tubulin, neurofilaments and glial filaments was used to study the effects of methylmercury on the differentiation of retinoic acid-induced embryonal carcinoma cells into neurons and astroglia and on the cytoskeleton of these neuroectodermal derivatives. Methylmercury did not prevent undifferentiated embryonal(More)
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