Elizabeth Dietter Schultz

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Cardiac myofibrillogenesis was examined in cultured chick cardiac cells by immunofluorescence using antibodies against titin, actin, tropomyosin, and myosin. Primitive cardiomyocytes initially contained stress fiber-like structures (SFLS) that stained positively for alpha actin and/or muscle tropomyosin. In some cases the staining for muscle tropomyosin and(More)
Skeletal muscle satellite cells were isolated from soleus and extensor digitorum longus muscles of Sprague-Dawley rats at selected ages between 6 days and 30 months and grown in cell culture. Cells at each donor age were monitored individually to determine the number of progeny they were capable of producing. Under identical in vitro conditions, the average(More)
Rattlesnake body and shaker muscles were studied using light microscopy and histochemistry. Five myofiber types are distinguishable in the body musculature. The majority are large diameter fast twitch fibers with high alkaline-stable ATPase activity and few mitochondria. In the shaker muscle the major fiber differs from all body fibers in that myofibrils do(More)
Satellite cells quantitated in the rat soleus and extensor digitorum longus (EDL) muscles following a complete regeneration returned to "normal" percentages of myofiber nuclei in both muscles 3 months after injury. Following cross-transplantation, the percentage of satellite cell nuclei in the EDL regenerated in the soleus bed was indistinguishable from the(More)
Satellite cells exhibit a number of distinct morphological changes after denervation which appear to be a direct response to nerve section or altered functional state of the muscle fibers. These changes appear generally related to increased movement and overall activation of the cells. After longer periods of denervation many satellite cells appear to(More)
Skeletal muscle satellite cells are myogenic stem cells that function to repair damaged muscle fibers. Participation of satellite cells in a regeneration response following muscle injury results in a significant reduction in their cumulative proliferation potential. The magnitude of the reduction is proportional to the number of regeneration responses in(More)
Fiber type classification of p-phenylene-diamine-stained transverse sections of rat skeletal muscle correlated with electron microscopic observations demonstrate that satellite cells are not equally distributed among the various fiber types in either the EDL or soleus at 1 and 12 months of age. Of the three fiber types in the EDL, satellite cells are(More)
After the transplantation of rat primary muscle cells into the caudate or cortex of recipient rats, the muscle cells were able to persist for at least 6 months. Muscle cells transfected with expression plasmids prior to transplantation were able to express reporter genes in the brains for at least 2 months. These results suggest that muscle cells might be a(More)