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OBJECTIVES This study aimed to determine the presence of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in different environments. METHODS Clinical samples and stool samples from animal farms, sewage, human faecal carriers attending the emergency room and faecal carriers in the context of food-borne disease outbreaks were subcultured(More)
We evaluated the presence of various β-lactamase genes within the bacteriophages in sewage. Results showed the occurrence of phage particles carrying sequences of bla OXA-2 , bla PSE-1 or bla PSE-4 and bla PSE-type genes. Phages may contribute to the spread of some β-lac-tamase genes. B acteriophages provide one of the most efficient vehicles for moving DNA(More)
The purpose of this investigation was to determine the prevalence of plasmid-mediated AmpC (pAmpC) and carbapenemases in Enterobacteriaceae collected from 35 hospitals in Spain and to establish their epidemiological relationships. We conducted a prospective multi-centre study on pAmpC- or carbapenemase-producing Enterobacteriaceae isolates from clinical(More)
OBJECTIVES To gain insights into ampC transmission between bacterial strains. METHODS We examined the genetic context of 117 acquired ampC genes from 27119 Enterobacteriaceae collected between 1999 and 2007. Plasmid analysis was carried out by PCR-based replicon or relaxase typing, S1-PFGE and Southern hybridization. I-CeuI/PFGE was used for isolates not(More)
LETTERS land. Furthermore, Ct. felis often feeds on humans. Clinicians encountering patients with fever or rash (or both) and a history of cat contact or flea bites should consider a diagnosis of R. felis. Laboratory confirmation of infection is not easy, but in vitro culture of R. felis, and hence material for a sero-logic assay for the diagnosis of human(More)
OBJECTIVES We describe 12 VIM-1-producing strains (7 Enterobacter cloacae, 2 Klebsiella pneumoniae and 3 clonal Klebsiella oxytoca strains) detected among clinically relevant Enterobacteriaceae isolates from routine cultures at the Hospital del Mar (Barcelona, Spain) from December 2006 to May 2007. METHODS Susceptibility to carbapenems was evaluated with(More)
led to the detection of Salmonella enterica and extended-spectrum cephalosporin-resistant Escherichia coli (ESCREC). Stool cultures were performed for 22 symptomatic campers, three asymptomatic food handlers, and 10 healthy household members. Of the 22 campers, 19 had Salmonella enterica, 9 had an ESCREC strain carrying an extended-spectrum β-lactamase, and(More)
A between-species comparison of antimicrobial resistance in enterobacteria in fecal flora. Sir, In recent years, extended-spectrum b-lactamases (ESBLs) have been detected extensively among Enterobacteriaceae isolates on all inhabited continents. 1 – 4 Strains carrying the recently emerged CTX-M-15 enzyme have caused several reported outbreaks involving(More)
OBJECTIVES To characterize the vectors involved in the dissemination of bla(CMY-2) genes in clinical isolates of Proteus mirabilis collected between 1999 and 2007. METHODS Plasmid analysis of 19 P. mirabilis carrying ampC genes was performed by PCR-based replicon typing, S1-PFGE and Southern hybridization with ampC and replicon probes. Isolates that could(More)
OBJECTIVES To describe the prevalence and risk factors for infection due to AmpC β-lactamase-producing Escherichia coli (AmpC-EC). METHODS For the prevalence study, all clinical isolates of E. coli with reduced susceptibility to third-generation cephalosporins were prospectively included from June 2010 to November 2011. For risk factor analysis, a(More)