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The poor solubility of polyene antibiotics in aqueous media limits their application in the therapy of systemic fungal infections. In the present paper we have demonstrated that the ionic state (net electrical charge) of the antibiotic molecule is an important factor in determining the aggregation and solubility properties of amphotericin B and its(More)
A comparative conformational analysis of cholesterol and ergosterol has been carried out using molecular mechanics methods. These studies are aimed at giving a better understanding of the molecular nature of the interaction of these sterols with polyene macrolide antibiotics. Structures of cholesterol and ergosterol determined by X-ray methods have been(More)
Glucosamine synthase (E.C. is a promising target in antifungal drug design. It has been reported that its potent inhibitor, N3-(4-methoxyfu-maroyl)-L-2,3-diaminopropanoic acid (FMDP), inactivates the enzyme by the Michael addition of the S-H group to the FMDP molecule followed by cyclisation reactions. In this study we have investigated, by means(More)
Continuing our theoretical studies of glucosamine synthase catalysis, we have carried out MNDO and ab initio calculations of the first stage of the reaction, which involves the attack of a cysteine thiol group from the enzyme active site on the side chain carboxyamide group of glutamine, producing ammonia and thioester. The reactants were modelled by methyl(More)
Plasma membranes were purified from spinach (Spinaceae oleracea L.) leaves by aqueous twophase partitioning. The NADH-hexaeyanoferrate(III) (HCF) oxidoreduetase (ORase) was released from the membrane by CHAPS solubilization and purified 360-fold by ion-exchange chromatography followed by affinity chromatography and size exclusion chromatography on FPLC. A(More)
Carbon fiber reinforced polymer (CFRP) laminates exhibit limited fracture toughness due to characteristic interlaminar fiber-matrix cracking and delamination. In this article, we demonstrate that the fracture toughness of CFRP laminates can be improved by the addition of multi-walled carbon nanotubes (MWCNTs). Experimental investigations and numerical(More)
The effect of different concentrations of lead was investigated in two different ways in the leaves of Helianthus annuus plants. In the first way, removed leaf discs from 5-wceks old plants were floated in 0.05, 0.25, 2.50 and 5.00 mM PbNO3 solutions for 2, 4 and 6 days. In addition, the chlorophyll content and the activity of peroxidase enzyme which are(More)
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