Elena Sveshnikova

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The regulatory subunit type II (RII) of cAMP-dependent protein kinase purified from human brain was represented by two proteins with apparent molecular masses of 51-52 kD and 54 kD. Dephosphorylation of human RII containing 3 mol phosphate/mol protein did not change the electrophoretic pattern. One-dimensional peptide mapping of 51-52 kD and 54 kD proteins(More)
Guanine nucleotide exchange factors (GEFs) of the Dbl family activate Rho proteins and thereby participate in diverse signaling pathways involving this family of small GTPases. However, specific role of Dbl-GEFs in developmental processes, particularly cell differentiation, remains largely unexplored. Recently, a novel member of the Dbl family,(More)
Four types of responses to iontophoretic application of serotonin were found in neurons of the ventral aspect of the visceral ganglion ofHelix pomatia. The responses differed in size, duration, latent periods, habituation to serotonin, and response to the action of serotonin antagonists (D-tubocurarine, tryptamine, neostigmine). Each type of response was(More)
Four types of unit responses to iontophoretical serotonin (5-hydroxytrytamine) application were obtained on the ventral surface of the visceral ganglion in the hibernating Helix pomatia. The responses differed in sign, duration, latent period, reaction to serotonin antagonists (d-tubocurarine, tryptamine, neostigmine). It seems that every type of unit(More)
Regulatory subunits type II (RII) purified from human, pig and bovine brains were compared using II monoclonal antibodies (MoAb) against bovine brain RII. Bovine RII has at least 5 antigenic sites located in the N-terminal 1-110 residues. Immunochemical difference detected between human and animal RII was more pronounced than between pig and bovine RII.(More)
Technology of industrial production of an active pharmaceutical substance (APS) of the recombinant human granulocyte colony-stimulating factor (rhG-CSF) involved the use of a highly productive E. coli strain capable of biosynthesis of rhG-CSF in the form of inclusion bodies (IB). A method of strain cultivation has been described, and methods of IB(More)
Mouse monoclonal antibodies to the bovine brain regulatory subunit type II (RII) were produced and antibodies of 11 clones were characterized. We determined their subclass, affinity constants, competivity and influence on two RII functions, namely cAMP binding and inhibition of the catalytic subunit (C) activity. 4 clones produced antibodies that increased(More)
Immunochemical analysis of the cAMP-dependent protein kinase regulatory subunit type II was performed with the use of two rabbit antisera elicited to a free R-subunit from pig brain and to a RcAMP complex. Quantitative precipitation of the homogeneous antigen revealed six determinants on the R-molecule. Of these at least one is localized in the R-fragment(More)
The activity of cAMP-dependent histone kinases (HK) and cAMP-independent casein kinases (CK) as well as cAMP level were assessed in gastric mucosa affected by various forms of gastritis as well as in gastric tumor and adjacent normal tissue. CK activity was higher whereas that of HK and cAMP level were lower in tumor and at a distance of 5-20 cm from its(More)