Elena Potokina

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BACKGROUND Affymetrix high density oligonucleotide expression arrays are widely used across all fields of biological research for measuring genome-wide gene expression. An important step in processing oligonucleotide microarray data is to produce a single value for the gene expression level of an RNA transcript using one of a growing number of statistical(More)
The recent development of Affymetrix chips designed from assembled EST sequences has spawned considerable interest in identifying single-feature polymorphisms (SFPs) from transcriptome data. SFPs are valuable genetic markers that potentially offer a physical link to the structural genes themselves. However, most current SFP prediction methodologies were(More)
BACKGROUND A typical genetical genomics experiment results in four separate data sets; genotype, gene expression, higher-order phenotypic data and metadata that describe the protocols, processing and the array platform. Used in concert, these data sets provide the opportunity to perform genetic analysis at a systems level. Their predictive power is largely(More)
It is well known that Affymetrix microarrays are widely used to predict genome-wide gene expression and genome-wide genetic polymorphisms from RNA and genomic DNA hybridization experiments, respectively. It has recently been proposed to integrate the two predictions by use of RNA microarray data only. Although the ability to detect single feature(More)
The history of Norway spruce distribution in the East European Plain is discussed with regard to the results of the allele diversity survey of the mitochondrial Nad1 gene, which is maternally inherited, and the chloroplast trnT-trnF region, which is paternally inherited in spruce. The polymorphism of organelle DNAs was examined in 221 genotypes from 28(More)
Allelic combinations of the Vrn-H1, Vrn-H2, Vrn-H3, Ppd-H1, and Ppd-H2 genes were investigated with allele-specific molecular markers in 91 spring-barley cultivars recommended for use in Russia and Belarus. In a field experiment under the conditions of Northwest Russia, heading date evaluation was performed in these cultivars. Barley varieties with the(More)
Analysis of the genetic diversity of the spruce populations in the northwest of the European part of Russia using microsatellite markers revealed two relatively isolated genetic clusters. One of them is composed of trees that were identified as P. abies according to morphological traits. Another cluster unites spruces that were determined as P. obovata and(More)
Combinations of the Ppd-D1, Vrn-A1, Vrn-B1, and Vrn-D1 gene alleles were analyzed using allele-specific primers in 56 varieties and lines of common wheat that were selected in the Lukyanenko Agricultural Research Institute. The significant influence of the combination of these alleles on the time of the beginning of the heading of the studied varieties was(More)
Morphophysiological traits of spring wheat (Triticum aestivum L.) forms are studied in pot and field experiments with 18-, 12-, and 8-h photoperiods. Genes controlling photoperiod sensitivity (Ppd) and growth habit (Vrn) are determined in these forms by means of hybridological analysis and allele-specific molecular markers. Ultra-early forms are valuable(More)
Net blotch of barley (Hordeum vulgare L.), caused by Pyrenophora teres f. teres, and spot blotch, caused by Cochliobolus sativus, are the most widespread and harmful diseases in the geographic range of the crop. Barley breeding for resistance to these diseases should employ large genetic diversity. The 11_11067 SNP marker was revealed on chromosome 6H(More)