Elena G. Plotnikova

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We have cloned and characterized novel oxygenolytic ortho-dehalogenation (ohb) genes from 2-chlorobenzoate (2-CBA)- and 2,4-dichlorobenzoate (2,4-dCBA)-degrading Pseudomonas aeruginosa 142. Among 3,700 Escherichia coli recombinants, two clones, DH5alphaF'(pOD22) and DH5alphaF'(pOD33), converted 2-CBA to catechol and 2,4-dCBA and 2,5-dCBA to(More)
The strain Rhodococcus ruber P25 utilizes 4-chlorobiphenyl (4CB) and 4-chlorobenzoic acid (4CBA) as sole carbon and energy sources. 4CB degradation by washed cells of strain P25 was accompanied by transient formation of 4CBA, followed by its utilization and release of equimolar amounts of chloride ions into the medium. The strain R. ruber P25 possessed(More)
Degradation of aromatic hydrocarbons by the Rhodococcus wratislaviensis KT112-7 isolated from technogenic mineral waste products of the BKRU1 “Uralkalii” plant was investigated (Berezniki, Perm krai). The R. wratislaviensis KT112-7 was shown to utilize increased concentrations of o-phthalic (o-PA) (8 g/L) and benzoic (BA) (3.4 g/L) acids. The strain grows(More)
MALDI mass spectra were generated for the type strain of Arthrobacter crystallopoietes VKM Ac-1107T and for closely related (99.6–100% 16S rRNA gene similarity) halotolerant Arthrobacter strains, as well as for some other Arthrobacter species. Results of the cluster analysis of the spectra were in agreement with the genotypic characteristics of bacteria(More)
Bacterial isolates from soils contaminated with (chlorinated) aromatic compounds, which degraded biphenyl/chlorinated biphenyls (CB) and belonged to the genera Rhodococcus and Pseudomonas, were studied. Analysis of the 16S rRNA gene sequences was used to determine the phylogenetic position of the isolates. The Rhodococcus cells were found to contain(More)
The review summarizes the data on new directions in biosensor technologies based on whole bacterial cells. Biosensors for the monitoring of mono(poly)aromatic hydrocarbons and their chlorinated derivatives, which are constructed with genetically modified bacterial cells bearing a reporter gene fusion, are considered. The operating principle of these(More)
Dioxygenases induced during benzoate degradation by the actinobacterium Rhodococcus wratislaviensis G10 strain degrading haloaromatic compounds were studied. Rhodococcus wratislaviensis G10 completely degraded 2 g/liter benzoate during 30 h and 10 g/liter during 200 h. Washed cells grown on benzoate retained respiration activity for more than 90 days, and a(More)
Biphenyl 2,3-dioxygenase is the key enzyme involved in the bacterial destruction of biphenyl and polychlorinated biphenyls (PCBs), which are highly stable toxic compounds. The diversity of bphA1 genes encoding the biphenyl 2,3-dioxygenase a-subunit of biphenyl-degrading bacteria from the microbial community of the Bering Sea coastal sediments (the Anadyr(More)
Polychlorinated biphenyls (PCBs) are persistent organic pollutants. Biphenyl 2,3-dioxygenase (BDO) is a key enzyme that determines the range of PCBs oxidized by a bacterial strain. BDO subunit α (BphA1) plays an essential role in substrate recognition and binding. The genes for dioxygenases that hydroxylate aromatic rings were screened and analyzed(More)
We report the 5,728,255-bp draft genome sequence of Rhodococcus ruber P25, isolated from a soil polluted with halogenated aromatic compounds in the city of Perm, Russia. The strain degrades polychlorinated biphenyls and a broad range of aromatic compounds. It possesses genes that mediate the degradation of biphenyls/polychlorinated biphenyls, naphthalene,(More)