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We have isolated the gene for a protein designated CCA1. This protein can bind to a region of the promoter of an Arabidopsis light-harvesting chlorophyll a/b protein gene, Lhcb1*3, which is necessary for its regulation by phytochrome. The CCA1 protein interacted with two imperfect repeats in the Lhcb1*3 promoter, AAA/cAATCT, a sequence that is conserved in(More)
Light-harvesting antenna complexes transfer energy from sunlight to photosynthetic reaction centers where charge separation drives cellular metabolism. The process through which pigments transfer excitation energy involves a complex choreography of coherent and incoherent processes mediated by the surrounding protein and solvent environment. The recent(More)
Magnetic resonance imaging (MRI) can elucidate the interior structure of an optically opaque object in unparalleled detail but is ultimately limited by the need to enclose the object within a detection coil; acquiring the image with increasingly smaller pixels reduces the sensitivity, because each pixel occupies a proportionately smaller fraction of the(More)
In this paper, we collect a few fairly well known facts about the numerical range and assemble them into an effective algorithm for computing the numerical range of an n × n matrix. Particular attention is paid to the case in which a line segment is embedded in the boundary of the numerical range, a case in which multiplicity is present. The result is a(More)
Nuclear magnetic resonance (NMR) is a well-established analytical technique in chemistry. The ability to precisely control the nuclear spin interactions that give rise to the NMR phenomenon has led to revolutionary advances in fields as diverse as protein structure determination and medical diagnosis. Here, we discuss methods for increasing the sensitivity(More)
When the in vitro synthesized precursor of a light-harvesting chlorophyll a/b binding protein (LHCP) from Lemna gibba is imported into barley etiochloroplasts, it is processed to a single form. Both the processed form and the precursor are found in the thylakoid membranes, assembled into the light-harvesting complex of photosystem II. Neither form can be(More)
The precursor for a Lemna light-harvesting chlorophyll a/b protein (pLHCP) has been synthesized in vitro from a single member of the nuclear LHCP multigene family. We report the sequence of this gene. When incubated with Lemna chloroplasts, the pLHCP is imported and processed into several polypeptides, and the mature form is assembled into the(More)
Compressive sensing allows signals to be efficiently captured by exploiting their inherent sparsity. Here we implement sparse sampling to capture the electronic structure and ultrafast dynamics of molecular systems using phase-resolved 2D coherent spectroscopy. Until now, 2D spectroscopy has been hampered by its reliance on array detectors that operate in(More)
We demonstrate that a position correlation magnetic resonance imaging (MRI) experiment based on two phase encoding steps separated by a delay can be used for quantifying diffusion across a membrane. This method is noninvasive, and no tracer substance or concentration gradient across the membrane is required. Because, in typical membranes, the T1 relaxation(More)
The capability to fabricate nanoscale structures is a fundamental step toward realizing the promise and potential of nanotechnology. We report on precise manipulation and thermal processing using 100-nm polystyrene latex nanoparticles. This approach is illustrated by fabricating a three-dimensional nanostructure by using an AFM tip to position nanoparticles(More)
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