Ekkehart Wodtke

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The phospholipid composition, fatty acid pattern and cholesterol content are studied in mitochondria of red lateral muscle of carp acclimated to high and low environmental temperatures. The results of the experiments are: mitochondria from cold-acclimated carp contain higher proportions of ethanolamine phosphatides than mitochondria from warm-acclimated(More)
The acclimation temperature of carp does not affect the amount of cytochrome c oxidase per mg mitochondrial protein as revealed from the reduced-minus-oxidized difference spectra of red muscle mitochondria from cold- and warm-acclimated carp. There are no differences between cold- and warm-acclimated fish in the substrate binding properties of the enzyme as(More)
The lipid fraction of liver mitochondria has been studied in carp acclimated to high and low environmental temperatures. Evidence is provided for a temperature-induced lipid adaptation which might control membrane fluidity. This supports suggestions made in a recent communication on temperature-induced changes in the Arrhenius functions of mitochondrial(More)
The membrane order of liver endoplasmic reticulum (ER) membranes of 10 degrees C- and 30 degrees C-acclimated carp has been compared using the fluorescence polarization technique with DPH as probe. Membranes from cold-acclimated fish displayed lower polarizations than corresponding membranes from warm-acclimated fish, the difference compensating for 34-50%(More)
1. Evidence is presented for ‘breaks’ in the Arrhenius plots of succinate and cytochrome oxidase systems of mitochondria from carp liver and red epaxial muscle. 2. Arrhenius activation energies (E a) of succinate and cytochrome oxidase systems above and below the transition temperatures (T *) are about half those reported for homoiotherms (Table 3).(More)
1. Adult eels were acclimated to different temperatures (usually to 7 ~ C for a t least 21 days and to 25 ~ C for 12 days). Oxygen uptake by mitochondria isolated from liver and red muscle was measured polarographically. The procedures for the two tissues differ mainly in tha t : a) a proteolytie digestion preceded the homogenization step in the case of red(More)
Hepatic microsomal activities of acyl-CoA:cholesterol acyltransferase (ACAT) and 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase, rate-limiting enzymes in cholesterol esterification and cholesterol synthesis, and the concentration sand compartmentalization of esterified and unesterified cholesterol, were studied in carp acclimated to 10 and 30 degrees C.(More)
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