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Replication of the giant RNA genome of severe acute respiratory syndrome (SARS) coronavirus (CoV) and synthesis of as many as eight subgenomic (sg) mRNAs are mediated by a viral… Expand
ABSTRACT Expression of the exceptionally large RNA genomes of CoVs involves multiple regulatory mechanisms, including extensive proteolytic processing of the large replicase polyproteins, pp1a and… Expand
Reverse genetics, a technique used to engineer specific mutations into viral genomes, was first performed for DNA viruses, either by transfecting cells with plasmids encoding the viral genome or by… Expand