Ekaterina G. Kurella

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1. The authors compare oxidative injury to brain and kidney Na/K-ATPase using in vitro and in vivo approaches. The substrate dependence of dog kidney Na/K-ATPase was examined both before and after partial hydrogen peroxide modification. A computer simulation model was used for calculating kinetic parameters. 2. The substrate dependence curve for the(More)
In the present work, we studied the effects of phenoxyl radicals, generated by tyrosinase-catalyzed oxidation of a phenolic antitumor drug, Etoposide (VP-16), on a purified dog kidney Na+/K(+)-ATPase by characterizing interactions of VP-16 phenoxyl radicals with the enzyme's SH-groups by ESR and correlating the loss of the enzymatic activity with the(More)
Brain Na,K-ATPase is subjected to direct modifying influence of the hypochlorite anion and hydrogen peroxide resulting in irreversible inactivation of the enzyme. The K0.5 values for these compounds are 20-40 microM and 1 mM, respectively. The inhibiting effect of hydrogen peroxide is suppressed by desferrioxamine (by 20-30%) which indicates that the(More)
The difference in the efficiency of carnosine as an antioxidant was found to be explained both by the source of carnosine and the specificity of models used to achieve visualization. Commercial carnosine samples were contaminated with compound (s) absorbing at 255-332 nm. At the same time they possessed better antioxidant activity in the models with(More)
In order to elucidate the biochemical roles of imidazol-containing dipeptides, we have studied quenching of singlet molecular oxygen (1O2) by carnosine (beta-alanyl-L-histidine), its structural components (L-histidine, imidazole, and beta-alanine), and related natural free-radical scavengers-L-anserine (beta-alanyl-1-methyl-histidine), ergothioneine(More)
Among the many biological effects of the natural histidine-containing dipeptide, carnosine [12, 7], its ability to protect the body against the action of radiation is noteworthy [13, 5]. The radioprotective effect of carnosine may have a number of different causes. One of them, put forward by Goncharenko and Kudryashov [1], might be connected with the(More)
It was shown that intake of carnosine in a dose of 50-100 mg/kg of body weight before X-ray irradiation resulted in an increase of the survival of experimental mice. The protective effect of carnosine was manifested, when it was injected either before or after irradiation, but the effect was more pronounced in the case of shortening time between irradiation(More)