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  • Haruo Sekizaki, Kotaro Itoh, Masaaki Murakami, Eiko Toyota, Kazutaka Tanizawa
  • Chemistry, Medicine
  • Comparative biochemistry and physiology. Part B…
  • 2000 (First Publication: 1 November 2000)
  • An anionic trypsin from pyloric caeca of chum salmon (Oncorhynchus keta) was purified by ammonium sulfate and acetone fractionation followed by affinity chromatography, gel-filtration, and DEAE-anionContinue Reading
  • Eiko Toyota, Kenneth K.-S. Ng, +4 authors Michael N. G. James
  • Chemistry, Medicine
  • Journal of molecular biology
  • 2002 (First Publication: 1 November 2002)
  • The nucleotide sequence and crystal structure of chum salmon trypsin (CST) are now reported. The cDNA isolated from the pyloric caeca of chum salmon encodes 222 amino acid residues, the same numberContinue Reading
  • Eiko Toyota, Daisuke Iyaguchi, Haruo Sekizaki, Midori Tateyama, Kenneth K S Ng
  • Medicine, Chemistry
  • Acta crystallographica. Section D, Biological…
  • 2009 (First Publication: 1 July 2009)
  • Three anionic salmon trypsin isoforms (CST-1, CST-2 and CST-3) were isolated from the pyloric caeca of chum salmon (Oncorhynchus keta). The order of catalytic efficiency (K(m)/k(cat)) of the isoformsContinue Reading
  • Susumu Kawano, Daisuke Iyaguchi, Chiaki Okada, Yusuke Sasaki, Eiko Toyota
  • Biology, Medicine
  • The Protein Journal
  • 2013 (First Publication: 1 June 2013)
  • Attempts to obtain active E-selectin from Escherichia coli (E. coli) have not yet been successful. In this study, we succeeded in expressing the recombinant lectin and epidermal growth factor domainContinue Reading