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OBJECTIVE To evaluate the subjective estimation of noise-induced discomfort and its correlation to psychoacoustic and physiological parameters under laboratory conditions. To establish an effective description of sound qualities of road traffic noise, supplementing the current standards and calculation specifications. METHODS Pass-by vehicle noise samples(More)
Molecular detection of herpes simplex virus (HSV) DNA is recognized as the reference standard assay method for the sensitive and specific diagnosis of central nervous system infections caused by HSV. In this study, a molecular assay based on real-time PCR on the LightCycler (LC) instrument was evaluated and compared with a home-brew molecular assay. The(More)
Elsas LJ II. Cystathionine-␤-synthase deficiency: detection of heterozygotes by the ratios of homocysteine to cysteine and folate. Kinetic PCR analysis by real-time monitoring of DNA amplification was first described 8 years ago (1). Recently, the LightCycler TM instrument (Roche Molecular Bio-chemicals, Mannheim, Germany), which allows high-speed thermal(More)
The AMPLICOR Enterovirus Test was evaluated with 103 cerebrospinal fluid (CSF) specimens. Twenty-seven CSF specimens were culture positive. With the AMPLICOR test, enterovirus RNA was detected in 34 specimens. Compared with culture, the AMPLICOR test gave a sensitivity of 96.3% and a specificity of 100%. The sensitivity of culture was 79.4% in comparison(More)
Molecular detection of Bordetella pertussis DNA is a sensitive and specific method for the rapid diagnosis of pertussis. In this study, a new molecular assay for the detection and differentiation of Bordetella spp. based on automated DNA extraction and real-time PCR was evaluated. The analytical sensitivity of the new assay was determined by Probit analysis(More)
In this study, we established a fully automated molecular assay for qualitative detection of hepatitis C virus (HCV) in serum and whole-blood samples and compared it with conventional molecular assays, including manual HCV RNA extraction protocols. Whole-blood samples were collected from patients with and without chronic HCV infection in EDTA tubes and(More)
In this study, we compared serum hepatitis C virus (HCV) RNA concentrations with HCV RNA concentrations in whole blood collection tubes, including two different types of EDTA tubes and nucleic acid stabilization tubes (NASTs). We also investigated the impact of a processing delay on HCV RNA concentration in these tubes. In NASTs, the mean HCV RNA(More)
Candida biofilms on indwelling devices are an increasing problem in patients treated at intensive care units. The goal of this study was to examine the occurrence and frequency of these biofilms. A total of 172 catheters were collected from 105 male and 67 female patients (the age range of both patient groups was from 3 weeks to 98 years old). The catheters(More)
To the Editor: In many parts of the world, the proportions of methicil-lin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant en-terococci, and extended-spectrum β-lactamase (ESBL)–producing organisms in the family Enterobacteriaceae have increased remarkably during recent years (1). However, proportions of antimicrobial drug resistance vary(More)
peroxide generated during cellular insulin stimulation is integral to activation of the distal insulin-signaling cascade in 3T3–L1 adipocytes. Enhanced potential for oxidative stress in hyperinsulinemic rats: Imbalance between hepatic peroxisomal hydrogen peroxide production and decomposition due to hyperinsulinemia. Insulin signaling is inhibited by(More)