Efrem Alessandro Foglia

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Optical imaging through biological samples is compromised by tissue scattering and currently various approaches aim to overcome this limitation. In this paper we demonstrate that an all optical technique, based on non-linear upconversion of infrared ultrashort laser pulses and on multiple view acquisition, allows the reduction of scattering effects in(More)
Biomedical issues in vasculogenesis and cardiogenesis require methods to follow hemodynamics with high spatial (micrometers) and time (milliseconds) resolution. At the same time, we need to follow relevant morphogenetic processes on large fields of view. Fluorescence cross-correlation spectroscopy coupled to scanning or wide-field microscopy meets these(More)
OBJECTIVE The goal of this study was to determine the in vivo functions of the synaptic proteins neurexins and neuroligins in embryonic vascular system development using zebrafish as animal model. METHODS AND RESULTS In the present study, we show that the knockdown of the α-form of neurexin 1a induces balance defects and reduced locomotory activity,(More)
N eurexins (NRXNs) are a family of cell adhesion molecules involved in the modulation of synaptic activity. 1 In mammals, there are 3 different NRXN genes, each presenting 2 alternative promoters which drive the synthesis of 2 major isoforms per gene (a long a-NRXN, and a short C-terminal b-NRXN) 2 whereas a further degree of heterogeneity is obtained(More)
During mouse skeletal muscle development, the Nfix gene has a pivotal role in regulating fetal-specific transcription. Zebrafish and mice share related programs for muscle development, although zebrafish develops at a much faster rate. In fact, although mouse fetal muscle fibers form after 15 days of development, in fish secondary muscle fibers form by 48(More)
Muscle regulatory factors activate myogenesis in all vertebrates, but their role has been studied in great detail only in the mouse embryo, where all but myogenin--Myod, Myf5 and Mrf4--are sufficient to activate (albeit not completely) skeletal myogenesis. In the zebrafish embryo, myod and myf5 are required for induction of myogenesis because their(More)
T he lysophospholipid sphingosine-1-phosphate (S1P) is a signaling molecule that acts through the binding to a family of 7-transmembrane G-protein–coupled receptors that include 5 high-affinity members (S1P 1–5). 1 S1P receptors modulate a variety of biological processes, including cell adhesion , migration, and endocytosis with different and sometimes(More)
BACKGROUND Endothelial cell junctions control blood vessel permeability. Altered permeability can be associated with vascular fragility that leads to vessel weakness and haemorrhage formation. In vivo studies on the function of genes involved in the maintenance of vascular integrity are essential to better understand the molecular basis of diseases linked(More)
OBJECTIVE Endothelial sphingosine-1-phosphate (S1P) receptor-1 (S1P(1)) affects different vascular functions, including blood vessel maturation and permeability. Here, we characterized the role of the zS1P(1) ortholog in vascular development in zebrafish. METHODS AND RESULTS zS1P(1) is expressed in dorsal aorta and posterior cardinal vein of zebrafish(More)
Microscopy techniques can readily visualize the finest details of embryo vasculature, but still lack to provide a complete three-dimensional representation of blood flow parameters. We present an in-vivo 3D imaging technique, able to reconstruct the blood cell velocity vector over a large volume of zebrafish embryos. This low cost and relatively simple(More)